Наборы для ПЦР

Store at -20°C, avoid frequent thawing and freezing

High Fidelity Pol (2.5 units/µl)
dNTP Mix
High Fidelity Buffer - 10 x conc.

High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.

Store at -20°C, avoid frequent thawing and freezing

High Fidelity Hot Start Pol (2.5 units/µl)
dNTP Mix
High Fidelity Buffer - 10 x conc.

High Fidelity Hot Start Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.

Store at -20°C, avoid frequent thawing and freezing

Hot Start Pol (5 units/µl)
dNTP Mix
Hot Start Buffer complete - 10 x conc.
MgCl₂ Stock Solution

Hot Start Core Kit provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
Hot Start Pol requires no prolonged activation step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Hot Start Core Kit - red contains Hot Start Pol - red for easier handling and to facilitate the preparation of the master mix.

Store at -20°C, avoid frequent thawing and freezing

Pfu-X Polymerase (2.5 units/µl)
dNTP Mix
Pfu-X Buffer - 10 x conc.

Pfu-X Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.

Store at -20°C, avoid frequent thawing and freezing

Taq Pol (5 units/µl)
dNTP Mix
Taq Reaction Buffer complete - 10 x conc.
MgCl₂ Stock Solution

Taq Core Kit is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The buffer system is recommended for plate based PCR and automated pipetting.
Taq Core Kit - red contains Taq Pol - red for easier handling and to facilitate the preparation of the master mix.

Store at -20°C, avoid frequent thawing and freezing

Taq Pol (5 units/µl)
dNTP Mix
High Yield Buffer complete - 10 x conc.
MgCl₂ Stock Solution

Taq Core Kit / high yield is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives best results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
Taq Core Kit - red / high yield contains Taq Pol - red for easier handling and to facilitate the preparation of the master mix.