Добавки и буферы для ПЦР

Store at -20°C in the dark

100x concentration

EvaGreen^® Fluorescent Gel Stain is a superior DNA staining dye for DNA analysis in gel electrophoresis applications. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent allowing the detection of lowest DNA amounts. It is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

Store at -20 °C

6x concentration

Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels.
Blue Gel Loading Buffer contains bromophenol blue and xylene cyanol FF as tracking dyes and indicator for DNA fragment migration. It is recommended for loading of fragment larger than 500 bp.
Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and is recommended for loading of fragment from 100 to 2000 bp.
Orange Gel Loading Buffer contains orange G and is recommended for loading of fragment smaller than 500 bp.

Store at -20 °C in the dark

6x concentration

Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen^® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Its high quantum yield and excellent stability makes it the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR^® Green.
The buffers contain tracking dyes as indicators for DNA fragment migration.
The Gel Loading Buffers provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR^® Green.

Store at 2-8°C or -20°C

Taq-stabilizer
GC-enhancer

PCR Additives Kit contains a stabilizer and enhancer bundle for the amplification of difficult templates such as GC-rich or extremly long fragments.
5-10% Taq-stabilizer in the PCR reaction is recommended to improve amplification efficiency and specificity. It stabilizes Taq polymerase and reduces secondary DNA structures.
10-20% GC-enhancer in the PCR is recommended for amplifying GC-rich templates. It reduces the melting temperature of DNA.

Store at -20°C

beta-actin Primer Mix
Human genomic DNA (male)

PCR Control Kit is designed for quality and benchmark tests, as positive control or internal lab standard for Taq DNA polymerases. The kit contains a forward / reverse primer-pair designed to amplify a 295 bp long fragment of the β-actin gene from human genomic DNA.

Store at 2-8°C or -20°C

PCR-grade Water

PCR-grade Water is ultrapure, sterile filtered and autoclaved water for use in molecular biology applications like standard PCR, qPCR or RT-PCR. It is treated with DEPC to avoid any residual enzymatic activity.