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Смеси для количественной ПЦР с флуоресцентным зондом с двойной меткой qPCR Mixes for Dual Labeled Fluorescent Probes
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Серия qPCR Master разработана для real-time анализа образцов ДНК, используя детекцию, основанную на ДНК-зондах. Смеси рекомендованы для использования с двойной флуоресцентной меткой, например, TaqMan®, молекулярными маяками или FRET - зондами. Они обеспечивают простой в обращении и мощный инструмент для количественного анализа образцов ДНК в широком динамическом диапазоне до 6 порядгов с исключительной чувствительностью и точностью.
Мастер-миксы содержат все реагенты, необходимые для количественной ПЦР (кроме матрицы, праймеров и флуоресцентного зонда) в виде 2-x концентрированного готового к использованию раствора. Высокая специфичность и чувствительность смеси достигается с помощью оптимизированной полимеразы с горячим стартом. Ее активность блокирована при температуре окружающей среды и включается автоматически при начале первой денатурации. Термическая активация предотвращает удлинение неспецифически связавшихся праймеров и димеров праймеров при низких температурах в течение подготовки ПЦР.
Смеси qPCR ProbesMaster, содержащие или не содержащие UNG, доступны в сочетании с low ROX и high ROX как эталонного красителя для нормализации и корректировки сигнала внутри термоциклера. .
Версия голубого окраса смеси qPCR GreenMaster mixes содержит инертный голубой краситель для более простого обращения. Она особо рекомендуется для облегчения дозирования в сочетании с белым или прозрачным пластиком
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster, 2x conc. PCR-grade water |
qPCR ProbesMaster is optimized for block-based PCR instruments.
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Datasheet
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster with highROX must be stored in the dark qPCR ProbesMaster with highROX, 2x conc. PCR-grade water |
qPCR ProbesMaster with highROX contains 500 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | ||||||||||||||||||
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster with lowROX must be stored in the dark qPCR ProbesMaster with lowROX, 2x conc. PCR-grade water |
qPCR ProbesMaster with lowROX contains 50 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | ||||||||||||||||||
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster with UNG, 2x conc. PCR-grade water |
qPCR ProbesMaster with UNG contains UNG (Uracil-N-Glycosylase).
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. The reaction chemistry of the kit is optimized for block-based PCR instruments. | ||||||||||||||||||
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster with UNG/highROX must be stored in the dark qPCR ProbesMaster with UNG/highROX, 2x conc. PCR-grade water |
qPCR ProbesMaster with UNG/highROX contains UNG (Uracil-N-Glycosylase) and 500 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | ||||||||||||||||||
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Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible qPCR ProbesMaster with UNG/lowROX must be stored in the dark qPCR ProbesMaster with UNG/lowROX, 2x conc. PCR-grade water |
qPCR ProbesMaster with UNG/lowROX contains UNG (Uracil-N-Glycosylase) and 50 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | ||||||||||||||||||
Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
