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mRNA Modification


Synthetic messenger RNA (mRNA) can be used to deliver exogenous genetic information inside cells that is converted by the cellular translational machinery into the encoded protein which subsequently induces a cellular response depending on its biological function.

Therapeutic protein encoding mRNAs have most recently gained increasing attention as versatile protein delivery molecules in non-viral gene therapy mostly due to their superior safety profile compared to plasmid DNA-based approaches (inability to integrate into the host genome)[1]. In addition, high-purity mRNA is easily synthesized even large scales by phage RNA polymerase-mediated in vitro transcription[2,3].

Their application however, was so far been hampered by the limited stability and strong immunogenicity of in vitro transcribed mRNAs subsequently leading to increased expression rates of the respective protein.

Enzymatic incorporation of a set several nucleotide analogs (single or combined) during the transcription process markedly improves the pharmacokinetic properties of in vitro transcribed mRNA both in terms of stability (increased nuclease resistance) and decreased immunogenicity (Fig. 1)[4-8].

Find more Cap analogs in the 5'-Capping section!
 
s2UTPm6ATPm5CTPARCAΨin vitro TranscriptionmRNA Modification
 
Figure 1: Pharmacokinetic properties of synthetic mRNA are improved by nucleotide analog incorporation during in vitro RNA synthesis. ARCA: "anti-reverse" cap analog, m5CTP = 5'-methyl-cytidine triphosphate, m6ATP=N6-methyl-adenosine-5'-triphosphate, s2UTP = 2-thio-uridine triphosphate, Ѱ = pseudouridine triphosphate.
 

For bulk inquiries please contact us at nucleotides@jenabioscience.com
 

Datasheet
НазваниеКодЦена Количество
L pack NU-1151L 27059.27 руб. 5 x 10 µl
(100 mM)
S pack NU-1151S 7423.38 руб. 10 µl
(100 mM)



Datasheet
НазваниеКодЦена Количество
L pack NU-1138L 44504.25 руб. 5 x 10 µl
(100 mM)
S pack NU-1138S 15193.19 руб. 10 µl
(100 mM)



Datasheet
НазваниеКодЦена Количество
1 mg pack NU-855-1 13867.49 руб. 1 mg
5 mg pack NU-855-5 62689.25 руб. 5 mg



НазваниеКодЦена Количество
L pack NU-855L 48182.07 руб. 5 x 10 µl
(100 mM)
S pack NU-855S 10706.75 руб. 10 µl
(100 mM)



Datasheet
НазваниеКодЦена Количество
L pack NU-1101L 27059.27 руб. 5 x 10 µl
(100 mM)
S pack NU-1101S 7423.38 руб. 10 µl
(100 mM)



Datasheet
НазваниеКодЦена Количество
L pack NU-1139L 50943.22 руб. 5 x 10 µl
(100 mM)
S pack NU-1139S 11287.27 руб. 10 µl
(100 mM)




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