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BseB I

Datasheet
НазваниеКодЦена Количество
L pack EN-108L 15849.17 руб. 5 x 4500 Units
S pack EN-108S 3962.51 руб. 4500 Units
Recognition Sequence:
5
Heat inactivation: No.

Ligation and recutting: After ten-fold overdigestion with BseB I,
<50 % of the DNA fragments can be ligated.

Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.

Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG 4000.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (70 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.





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