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Клетки эпителия молочной железы человека: HMEpC. Первичные клеточные линии Cell Applications.

Human Mammary Epithelial Cells: HMEpC

Клетки Эпителия Молочной Железы Человека (HMEpC) предоставляют прекрасную модельную систему для изучения многих аспектов эпителиальных функций и заболеваний, особенно связанных с  канцерогенезом.

HMEpC от Cell Applications, Inc. были использованы во многих исследованиях, например:

(Текст на языке оригинала)
  • Investigate the role of exosomes secreted by cancer cells in formation of tumor permissive microenvironment through manipulation of normal mammary epithelium
  • Serve as control in a study investigating antitumor properties of cannabinoids and stem cell microenvironment
  • Determine that differential expression of glycoproteins allows classification of human breast cells into normal, benign, malignant, basal, and luminal groups
  • Identify ALDH isoform 5A1 as a potential target for treatment of human breast ductal carcinoma
  • Determine that combination of an anti-growth factor treatment using phototherapy (UV-B) is more effective
  • Investigate cell survival following DNA damage
  • Show the important roles of tumor suppressors in mammary epithelial differentiation
  • Explain protection of extracellular matrix from degradation in normal mammary epithelia,
  • Show that Maspin loss in metastatic cancer leads to unrestricted ECM degradation, contributing to metastasis, and that loss of EcSOD expression also promotes invasiveness by disrupting ECM
  • Investigate the role of shortened telomeres in initiation of genomic instability, cytokinesis failure and polyploidy
  • Elucidate the role of Myc in malignancy by studying its ability to transform primary epithelial cells
  • Demonstrate, along with Human dermal Fibroblasts, also от Cell Applications, Inc., that resveratrol inhibits mono-ubiquitination of histone H2B

Характеристика: клетки имеют характерную морфологию в соответствии с происхождением эпителия и положительны на маркер эпителиальных клеток цитокератин 18.


Normal human mammary glands. Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
5th passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HMEpC(830-05a), 500 ml of Mammary Epithelial Cell Growth Medium (815-500), & a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Growth Medium at 6th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings



Pampalona, J., E. Roscioli, W. Silkworth, B. Bowden, A. Genesca, L. Tussell and D. Cimini. 2016. Chromosome Bridges Maintain Kinetochore-Microtubule Attachment throughout Mitosis and Rarely Break during Anaphase.  PLoS ONE 11(1):e0147420. doi:10.1371/journal.pone.0147420.
Armstrong, M., M. Stang, Y. Liu, J. Yan, E. Pizzoferrato, & J. Yim. 2015. IRF-1 inhibits NF-κB activity, suppresses TRAF2 and cIAP1 and induces breast cancer cell specific growth inhibition. Cancer Biology & Therapy,DOI:10.1080/15384047.2015.1046646.
Komatsu, N., Y. Fujita, M. Matsuda, and K. Aoki. 2015. mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogenic KRas-mutant cancer cells. Oncogene , 23 February | doi:10.1038/onc.2015.16
Khalkhali-Ellis, Z., W. Goossens, N. Margaryan, and M. Hendrix. 2014. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland. PLoS ONE, July 23.
Teoh-Fitzgerald M, M. Fitzgerald, W. Zhong, R. Askeland, and F. Domann. 2014. Epigenetic reprogramming governs EcSOD expression during human mammary epithelial cell differentiation, tumorigenesis and metastasis. Oncogene, 33:358-368.
Holtzhausen, A., C. Golzio, T. How, Y.-H. Lee, W.P. Schiemann, N. Katsanis, and G.C. Blobe. 2013. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development. The FASEB Journal. 28:1-20.
Sarkar, S., S. Rajput, A.K. Tripathi, and M. Mandal. 2013. Targeted therapy against EGFR and VEGFR using ZD6474 enhances the therapeutic potential of UV-B phototherapy in breast cancer cells. Molecular cancer. 12:122.
Teoh-Fitzgerald, M., M. Fitzgerald, W. Zhong, R. Askeland, and F. Domann. 2013. Epigenetic reprogramming governs EcSOD expression during human mammary epithelial cell differentiation, tumorigenesis and metastasis. Oncogene.doi: 10.1038/onc.2012.582.
Timpe, L.C., R. Yen, N.V. Haste, C. Litsakos-Cheung, T.-Y. Yen, and B.A. Macher. 2013. Systemic alteration of cell-surface and secreted glycoprotein expression in malignant breast cancer cell lines. Glycobiology. 23:1240-1249.
Yen, T.-Y., S. Dutta, C. Litsakos-Cheung, A. Corona, L. Timpe, and B. Macher. 2013. Overcoming Challenges and Opening New Opportunities in Glycoproteomics. Biomolecules. 3:270-286.
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2012. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent US 8106004 B2.
Kaur, H., S. Mao, Q. Li, M. Sameni, S.A. Krawetz, B.F. Sloane, and R.R. Mattingly. 2012. RNA-Seq of Human Breast Ductal Carcinoma In Situ Models Reveals Aldehyde Dehydrogenase Isoform 5A1 as a Novel Potential Target. PloS one. 7:e50249.
Pampalona, J., C. Frías, A. Genescà, and L. Tusell. 2012. Progressive telomere dysfunction causes cytokinesis failure and leads to the accumulation of polyploid cells. PLoS genetics. 8:e1002679.
Teoh-Fitzgerald, M.L.T., M.P. Fitzgerald, T.J. Jensen, B.W. Futscher, and F.E. Domann. 2012. Genetic and Epigenetic Inactivation of Extracellular Superoxide Dismutase Promotes an Invasive Phenotype in Human Lung Cancer by Disrupting ECM Homeostasis. Molecular Cancer Research. 10:40-51.
Gao, Z., M.S. Xu, T.L. Barnett, and C.W. Xu. 2011. Resveratrol induces cellular senescence with attenuated mono-ubiquitination of histone H2B in glioma cells. Biochemical and biophysical research communications. 407:271-276.
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2011. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent Application US 20130102541 A1.
Yen, T.-Y., B.A. Macher, C.A. McDonald, C. Alleyne-Chin, and L.C. Timpe. 2011. Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of proteome research. 11:656-667.
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2010. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent Application US 20100273707 A1.
Soler, D., J. Pampalona, L. Tusell, and A. Genescà. 2009. Radiation sensitivity increases with proliferation-associated telomere dysfunction in nontransformed human epithelial cells. Aging Cell. 8:414-425.
Thibodeaux, C., X. Liu, G. Disbrow, Y. Zhang, J. Rone, B. Haddad, and R. Schlegel. 2009. Immortalization and transformation of human mammary epithelial cells by a tumor-derived Myc mutant. Breast Cancer Res Treat. 116:281-294.
Bailey, C.M., D.E. Abbott, N.V. Margaryan, Z. Khalkhali-Ellis, and M.J.C. Hendrix. 2008. Interferon Regulatory Factor 6 Promotes Cell Cycle Arrest and Is Regulated by the Proteasome in a Cell Cycle-Dependent Manner. Mol. Cell. Biol. 28:2235-2243.
Khalkhali-Ellis, Z., D.E. Abbott, C.M. Bailey, W. Goossens, N.V. Margaryan, S.L. Gluck, M. Reuveni, and M.J.C. Hendrix. 2008. IFN-γ regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells. J. Cell. Biochem. 105:208-218.
Postovit, L.-M., N.V. Margaryan, E.A. Seftor, D.A. Kirschmann, A. Lipavsky, W.W. Wheaton, D.E. Abbott, R.E.B. Seftor, and M.J.C. Hendrix. 2008. Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells. Proceedings of the National Academy of Sciences. 105:4329-4334.
Tusell, L., D. Soler, M. Agostini, J. Pampalona, and A. Genescà. 2008. The number of dysfunctional telomeres in a cell: one amplifies; more than one translocate. Cytogenetic and Genome Research. 122:315-325.
Khalkhali-Ellis, Z., and M.J.C. Hendrix. 2007. Elucidating the Function of Secreted Maspin: Inhibiting Cathepsin D–Mediated Matrix Degradation. Cancer research. 67:3535-3539.
Wozniak, R., W. Klimecki, S. Lau, Y. Feinstein, and B. Futscher. 2007. 5-Aza-2'-deoxycytidine-mediated reductions in G9A histone methyltransferase and histone H3 K9 di-methylation levels are linked to tumor suppressor gene reactivation. Oncogene, 26:77-90.
Ligresti, A., A.S. Moriello, K. Starowicz, I. Matias, S. Pisanti, L. De Petrocellis, C. Laezza, G. Portella, M. Bifulco, and V. Di Marzo. 2006. Antitumor activity of plant cannabinoids with emphasis on the effect of cannabidiol on human breast carcinoma. The Journal of pharmacology and experimental therapeutics. 318:1375-1387.
Solomon, J.M., R. Pasupuleti, L. Xu, T. McDonagh, R. Curtis, P.S. DiStefano, and L.J. Huber. 2006. Inhibition of SIRT1 Catalytic Activity Increases p53 Acetylation but Does Not Alter Cell Survival following DNA Damage. Mol. Cell. Biol. 26:28-38.

For general cryopreservation of most primary cells. Contains FBS & DMSO. 040-50 6228.74 руб.

HMEpC Total Kit: Media, Subculture Reagents & Cells, Adult 830K-05a 90691.07 руб.

Cryopreserved HMEpC 830-05a 72876.52 руб.

124 x 24-Well Rxns TF102K 46715.54 руб.
25 x 24-Well Rxns TF102KS 6222.62 руб.

Human Epidermal Growth Factor RP1026-100 9966.12 руб.
Human Epidermal Growth Factor RP1026-500 22423.6 руб.
Human Epidermal Growth Factor RP1026-1000 30520.69 руб.

Basal medium (contains no growth supplement).В  Add GS before use. 814-500 7225.42 руб.

All-in-one ready-to-use 815-500 12706.82 руб.

Basal medium & growth supplement sold together packaged separately 815K-500 13703.5 руб.

Added to Basal Medium to create Growth Medium 815-GS 6602.41 руб.

Proliferating HMEpC 831-25a 72876.52 руб.
Proliferating HMEpC 831-75a 96546.14 руб.
Proliferating HMEpC 831-6Wa 96546.14 руб.
Proliferating HMEpC 831-96Wa 110249.64 руб.

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.

Extended Family Products

100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.

Total RNA prepared from Human Mammary Epithelial Cells, adult 830-R10a 40486.8 руб.
Total RNA prepared from Human Mammary Epithelial Cells, adult 830-R25a 80974.29 руб.

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