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Фибробласты легких человека: HLF. Первичные клеточные линии Cell Applications.

Human Lung Fibroblasts: HLF

Фибробласты Легких Человека (HLF) обеспечивают прекрасную модель для изучения многих аспектов функций и патологий человеческих легких.

HLF от Cell Applications, Inc. были использованы во множестве исследований, например для демонстрации:

(Текст на языке оригинала)
  • Differential gene expression, cytokines, growth factors and interleukins
  • Receptor activation, inhibition and receptor-interacting protein
  • Cell behavior changes, including synthesis of collagen and other extracellular matrix molecules, as well as actin reorganization, collagen contraction and fibroblast / myofibroblast trans-differentiation.
  • Lung disease, disorders and injury, including hypoxia, tissue rigidity, pulmonary fibrosis and hypertension
  • That unwanted cell growth can be inhibited, and that the healing process can be understood and improved


Normal human lung parenchyma.  Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HLF (506-05a or 506-05f), 500 ml of Fibroblast Growth Medium (116-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings.




Peng, H., W. Gao, F. Chong, H. Liu, and J. Zhang. 2015. Semaphorin 4A enhances lung fibrosis through activation of Akt via PlexinD1 receptor. J Biosci, DOI 10.1007/s12038-015-9566-9.
Nyp, M., A. Navarro, M. Rezaiekhaligh, R. Perez, S. Mabry, and I. Ekekezie. 2014. TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation. Respiratory Research. 15:19.
Ni, J., Z. Dong, W. Han, D. Kondrikov, and Y. Su. 2013. The role of RhoA and cytoskeleton in myofibroblast transformation in hyperoxic lung fibrosis. Free Radical Biology and Medicine. 61:26-39.
Buck, M., and M. Chojkier. 2011. C/EBPβ-Thr217 Phosphorylation Signaling Contributes to the Development of Lung Injury and Fibrosis in Mice. PloS one. 6:e25497.
Zheng, M., D.M. Jones, C. Horzempa, A. Prasad, and P.J. McKeown-Longo. 2011. The first type III domain of fibronectin is associated with the expression of cytokines within the lung tumor microenvironment. Journal of Cancer. 2:478.
Navarro, A., M. Rezaiekhaligh, J.A. Keightley, S.M. Mabry, R.E. Perez, and I.I. Ekekezie. 2009. Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts. American Journal of Physiology - Lung Cellular and Molecular Physiology. 296:L928-L935.
Okada, M., K. Suzuki, M. Matsumoto, K. Takada, T. Nakanishi, H. Horikoshi, T. Higuchi, Y. Hosono, M. Nakayama, and F. Ohsuzu. 2009. Effects of angiotensin on the expression of fibrosis-associated cytokines, growth factors, and matrix proteins in human lung fibroblasts. Journal of Clinical Pharmacy and Therapeutics. 34:289-299.
Tofovic, S.P., X. Zhang, E.K. Jackson, H. Zhu, and G. Petrusevska. 2009. 2-methoxyestradiol attenuates bleomycin-induced pulmonary hypertension and fibrosis in estrogen-deficient rats. Vascular pharmacology. 51:190-197.
Tofovic, S.P., X. Zhang, H. Zhu, E.K. Jackson, O. Rafikova, and G. Petrusevska. 2008. 2-Ethoxyestradiol is antimitogenic and attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling. Vascular pharmac. 48:174-183.
Ikeda, J.,, Y. Okada, H., Sakai, and H. Osuga. 2003. Anti-neurodegenerative agents. Patent Applications US 20050261306 A1.
Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.

HLF Total Kit: Media, Subculture Reagents & Cells, Adult 506K-05a 81347.96 руб.
HLF Total Kit: Media, Subculture Reagents & Cells, Fetal 506K-05f 81347.96 руб.

Cryopreserved HLF 506-05a 64779.43 руб.

Cryopreserved HLF 506-05f 64779.43 руб.

Human FGF9 ELISA Kit CL0348 52944.28 руб.

Basal medium (contains no growth supplement).В  Add GS before use. 115-500 6602.41 руб.

Basal medium & growth supplement sold together packaged separately 116K-500 13703.5 руб.

Added to Basal Medium to create Growth Medium 116-GS 5855.07 руб.

Total RNA prepared from human lung tissue 1H40-50 16039.28 руб.
Total RNA prepared from human lung tissue 1H40-250 60419.04 руб.

Proliferating HLF 507-96Wa 103397.89 руб.
Proliferating HLF 507-25a 64779.43 руб.
Proliferating HLF 507-75a 88448.37 руб.
Proliferating HLF 507-6Wa 88448.37 руб.

Proliferating HLF 507-25f 64779.43 руб.
Proliferating HLF 507-75f 88448.37 руб.
Proliferating HLF 507-96Wf 103397.89 руб.
Proliferating HLF 507-6Wf 88448.37 руб.

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.

Extended Family Products

100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.

25 x 24-Well Rxns TF103KS 6222.62 руб.
250 x 24-Well Rxns TF103K 46715.54 руб.

Human Fibroblast Growth Factor acidic RP1029-50 22423.6 руб.
Human Fibroblast Growth Factor acidic RP1029-10 9343.11 руб.
Human Fibroblast Growth Factor acidic RP1029-1000 180634.8 руб.

Total RNA prepared from Human Lung Fibroblasts, fetal 506-R10f 40486.8 руб.
Total RNA prepared from Human Lung Fibroblasts, fetal 506-R25f 80974.29 руб.

Related Products

All-in-one ready-to-use 116-500 11460.8 руб.

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