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Стромальные клетки костного мозга человека: HMSC. Первичные клеточные линии Cell Applications.

Human Marrow Stromal Cells: HMSC

Стромальные Клетки Костного Мозга Человека (HMSC) имеют потенциал для поддержания мультипотентности и активно размножаться in vitro. Костный мозг – основной кроветворный орган, но в дополнение к гемапоэтическому росту и дифференциации, стромальные клетки костного мозга могут быть индуцированы для формирования клеток других соединительных тканей, таких как кость, хрящ и жировая ткань, как и клетки нейроэктодермального  (нейроны) и эндодермального (гепатоциты) происхождений.

HMSC от Cell Applications, Inc. были использованы в исследованиях:

(Текст на языке оригинала)
  • Effects of TGF-β1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions
  • The differentiation potential of stem cells
  • HLA expression profiles of bone marrow-derived and adipose-derived stem cells
  • Heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering

Параметры

Tissue:
Human bone marrow. Each lot is tested negative for HIV, Hepatitis B and Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HMSC (492-05f), 500 ml Human Marrow Stromal Cell Growth Medium (419-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings


Документы



Публикации

2015
Teng, N., Y. Liu, H. Wu, Y. Liu, J. Ho, and O. Lee. 2015. Promotion of Mesenchymal-to-Epithelial Transition by Rac1 Inhibition with Small Molecules Accelerates Hepatic Differentiation of Mesenchymal Stromal Cells. Tissue Engineering Part A. Online ahead of editing, Jan 27.
2014
Gadkari, R., L. Zhao, T. Teklemariam, and B. Hantash. 2014. Human embryonic stem cell derived-mesenchymal stem cells: an alternative mesenchymal stem cell source for regenerative medicine therapy. Regenerative Medicine, 9:453-465.
Hantash, B. 2014. Differentiation of mesenchymal stem cells into fibroblasts, compositions comprising mesenchymal stem cell-derived fibroblasts, and methods of using the same. Patent US8822215 B2.
Purandare, B., T. Teklemariam, L. Zhao, and B. Hantash. 2014. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow-and adipose-derived mesenchymal stem cells. Regenerative Medicine 9: 67-79.
Teklemariam, T., B. Purandare, L. Zhao, and B. Hantash. 2014. Inhibition of DNA methylation enhances HLA-G expression in human mesenchymal stem cells. BBRC 452:753-759.
2013
Purandare, B., T. Teklemariam, L. Zhao, and B.M. Hantash. 2013. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow- and adipose-derived mesenchymal stem cells. Regenerative Medicine. 9:67-79.
2012
Kato, Y., I. Hirata, and M. Kanawa. 2012. Animal Cell Culture Kit, Method for Culturing Animal Cells, Method for Selective Culture of Animal Cells and Cell Differentiation Method. Patent Application US 20140030804 A1.
2011
Hirota, Y., and M. Hakoda. 2011. Relationship between dielectric characteristic by DEP levitation and differentiation activity for stem cells. Key Engineering Materials. 459:84-91.
Park, J., H. Yoon, B. Yoo, Y. Kim, and Y. Shin. 2011. Method for the preparation of dermal papilla tissue employing mesenchymal stem cells. Patent US 8017390 B2.
Tedder, M.E., A. Simionescu, J. Chen, J. Liao, and D.T. Simionescu. 2011. Assembly and testing of stem cell-seeded layered collagen constructs for heart valve tissue engineering. Tissue engineering. Part A. 17:25-36.
Zhao, L., and B.M. Hantash. 2011. TGF-b1 Regulates Differentiation of Bone Marrow Mesenchymal Stem Cells. Vitamins and hormones 87:127-141.
2010
Tedder, M.E.B. 2010. Characterization and Evaluation of a Novel Tissue Engineered Aortic Heart Valve Construct. Clemson University, PhD dissertation.
Tedder, M., A. Simionescu, J. Chen, J. Liao, and D. Simionescu. 2010. Assembly and Testing of Stem Cell-Seeded Layered Collagen Constructs for Heart Valve Tissue Engineering. Tissue Engineering Part A., 17:25-36.
2006
Baron, U., I. Turbachova, A. Hellwag, F. Eckardt, K. Berlin, U. Hoffmüller, P. Gardina, and S. Olek. 2006.  DNA Methylation Analysis as a Tool for Cell Typing. Epigenetics 1:55-60.

Le Visage, C., S.W. Kim, K. Tateno, A.N. Sieber, J.P. Kostuik, and K.W. Leong. 2006. Interaction of human mesenchymal stem cells with disc cells: changes in extracellular matrix biosynthesis. Spine. 31:2036-2042.




Cryopreserved HMSC Total Kit
НазваниеКодЦена
HMSC Total Kit: Media, Subculture Reagents & Cells, Adult 492K-05a 100781.52 руб.
HMSC Total Kit: Media, Subculture Reagents & Cells, Fetal 492K-05f 98290.16 руб.



Cryopreserved HMSC, adult
НазваниеКодЦена
Cryopreserved HMSC 492-05a 81597.3 руб.



Cryopreserved HMSC, fetal
НазваниеКодЦена
Cryopreserved HMSC 492-05f 79105.26 руб.



HMSC RNA
НазваниеКодЦена
Total RNA prepared from Human Human Marrow Stromal Cells, adult 492-R10a 47338.55 руб.
Total RNA prepared from Human Human Marrow Stromal Cells, adult 492-R25a 94677.11 руб.
Total RNA prepared from Human Human Marrow Stromal Cells, fetal 492-R10f 47338.55 руб.
Total RNA prepared from Human Human Marrow Stromal Cells, fetal 492-R25f 94677.11 руб.



M-CSF
НазваниеКодЦена
Human Macrophage Colony Stimulating Factor RP1003-10 22423.6 руб.
Human Macrophage Colony Stimulating Factor RP1003-100 72253.51 руб.
Human Macrophage Colony Stimulating Factor RP1003-1000 616649.26 руб.



M-CSF ELISA Kit, Human
НазваниеКодЦена
Human M-CSF ELISA Kit CL0444 59173.7 руб.



Proliferating HMSC, adult
НазваниеКодЦена
Proliferating HMSC 493-25a 81597.3 руб.
Proliferating HMSC 493-75a 105266.24 руб.
Proliferating HMSC 493-6Wa 105266.24 руб.
Proliferating HMSC 493-96Wa 120215.07 руб.



Proliferating HMSC, fetal
НазваниеКодЦена
Proliferating HMSC 493-25f 79105.26 руб.
Proliferating HMSC 493-75f 102774.88 руб.
Proliferating HMSC 493-96wf 117723.71 руб.
Proliferating HMSC 493-6wf 102774.88 руб.



Subculture Reagent Kit
НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.



Extended Family Products

Anti-SCF
НазваниеКодЦена
Rabbit Stem Cell Factor Antibody CG1536 по запросу



Cyto-X Colorimetric Cell Counting Reagent
НазваниеКодЦена
100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.



SCF
НазваниеКодЦена
Human Stem Cell Factor RP1004-100 56059.33 руб.
Human Stem Cell Factor RP1004-10 22423.6 руб.
Human Stem Cell Factor RP1004-1000 367497.65 руб.



Related Products

Human Marrow Stromal Cell Basal Medium
НазваниеКодЦена
Basal HMSC medium (contains no growth supplement).В  Add GS before use. 418-500 7225.42 руб.
Serum-Free Basal HMSC Medium 418sf-500 11834.47 руб.
Xeno-Free Basal HMSC Medium 418xf-500 13080.49 руб.



Human Marrow Stromal Cell Growth Medium
НазваниеКодЦена
All-in-one ready-to-use HMSC Growth Medium 419-500 14077.17 руб.
HMSC Growth Medium without Antibiotics 419A-500 14077.17 руб.
Serum-Free HSMC Growth Medium 419sf-500 21177.58 руб.
Xeno-Free HMSC Growth Medium 419xf-500 23669.62 руб.



Human Marrow Stromal Cell Growth Medium Kit
НазваниеКодЦена
Basal HSMC medium & HSMC growth supplement sold together packaged separately 419K-500 15073.85 руб.
Serum-Free HMSC Growth Medium Kit 419sfK-500 21800.59 руб.
Xeno-Free HMSC Growth Medium Kit 419xfk-500 24291.95 руб.



Human Marrow Stromal Cell Growth Supplement
НазваниеКодЦена
Added to HMSC Basal Medium to create HMSC Growth Medium 419-GS 7848.43 руб.
Serum-Free HMSC Growth Supplement 419sf-GS 12457.48 руб.
Xeno-Free HMSC Growth Supplement 419xf-GS 13703.5 руб.




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