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Синовиальные фибробласты человека: HFLS. Первичные клеточные линии Cell Applications.

Human Fibroblast-Like Synoviocytes: HFLS

Синовиальные Фибробласты Человека (HFLS) обеспечивают прекрасную клеточную модель для изучения нормальной и патологической физиологии синовиоцитов и развития заболеваний суставов.

Нормальные клетки HFLS от Cell Applications, Inc. использовались для изучения:

  • TNFα – опосредованных изменений экспрессии генов.
  • Вклада TNF-подобного слабого индуктори апоптоза (TWEAK) в воспаление суставов путем индуцирования хемокинов и матриксной металлопротеазы-9, предполагая TWEAK как новую цель для терапии.
  • Роли miR-124a в патогенезе артритов.
  • C/EBPβ-регулирование металлопротеиназ и членов семейства в синовиоцитах, стимулированных IL-1β.
  • HMW-HA подавление ADAMTS4 mRNA и экспрессия белка через пути CD44, p38 MAPK и JNK .
  • Анти-воспалительная и противоревматическая активность различных соединений, таких как ингибиторы NF-κB, AGIX-4207, HA–метотрексат конъюгатов и буцилламина.
  • Реактивные артриты, вызванные хламидийной инфекцией.
  • Механизмы артритоподобного синдрома у пациентов, инфицированных вирусом Чикугунья (CHIK).
  • Использование бедной лейкоцитами, свободной от эритроцитов, богатой тромбоцитами плазмы в клинической ортопедии, поскольку лейкоциты и эритроциты вызывают гибель синовиоцитов и выделение медиаторов, стимулирующих воспаление.
  • Связывающий белок хряща и MAGP2 как маркеры для различения хондроцитов и синовиальных клеток.
  • Создание индуцированных плюрипотентных клеток (iPSC) с использованием ставшего классическим “коктейля Яманаки ”, открытие, за которое Dr. Shinya Yamanaka получил Нобелевскую премию в 2012 году.
  • Возбудители, иммунопатологические механизмы и пути сигнальной трансдукции, ведущии к воспалению суставов при ревматоидных артритах.
  • Сигнальная роль эстрогена в увеличении воспалений.
  • Противовоспалительные свойсва растительных соединений Синоменина, предлагаемых для лечения ревматоидных артритов.
  • Действие состава внеклеточного матрикса на прикрепление и миграцию клеток, связанной с функциями Т-клеток в воспаленных тканях.
  • Роль эндогенных ретровирусов человека (HERV) в развитии ревматоидных артритов.
  • Активированная экспрессия различных форм HERV, способствующая развитию симптомов ревматоидных артритов с помощью различных механизмов.

Параметры

Tissue:
Normal human synovial tissues. Each lot is tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HFLS (408-05a), 500 ml of Synoviocyte Growth Medium (415-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 5 doublings


Документы



Публикации


2015
Jie, L., R.Huang, W. Sun, S. Wei, Y. Chu, Q. Huang, and H. Du. 2015. Role of cysteine‑rich angiogenic inducer 61 in fibroblast‑like synovial cell proliferation and invasion in rheumatoid arthritis. Molecular Medicine Reports, 11:917-923.
McCoy, J., D. Walkenhorst, K. McCauley, H. Elaasar, J. Everett, and K. Mix. 2015. Orphan nuclear receptor NR4A2 induces transcription of the immunomodulatory peptide hormone prolactin. Journal of Inflammation, 12:13. DOI 10.1186/s12950-015-0059-2
2014
Braun, H.J., H.J. Kim, C.R. Chu, and J.L. Dragoo. 2014. The Effect of Platelet-Rich Plasma Formulations and Blood Products on Human Synoviocytes: Implications for Intra-articular Injury and Therapy. The American Journal of Sports Medicine, 10.1177/0363546514525593.
Calabro, A., M. Lauer, and V. Hascall. 2014. Treatment of extracellular matrix to reduce inflammation. Patent Application US 20140135256 A1.
Calabro, A., M. Lauer, and V. Hascall. 2014. Treatment of extracellular matrix to reduce inflammation. Patent US 8658593 B2.
2013
Kataoka, Y., W. Ariyoshi, T. Okinaga, T. Kaneuji, S. Mitsugi, T. Takahashi, and T. Nishihara. 2013. Mechanisms involved in suppression of ADAMTS4 expression in synoviocytes by high molecular weight hyaluronic acid. In Biochemical and biophysical research communications: 432. 580-585.
Matsumoto, Y., H. Ichihara, M. Hino, M. Umebayashi, and R. Ueoka. 2013. Therapeutic effects of hybrid liposomes without drugs for rheumatoid arthritis. Drug Delivery: doi:10.3109/10717544.10712013.10869635.
Phuklia, W., J. Kasisith, N. Modhiran, E. Rodpai, M. Thannagith, T. Thongsakulprasert, D.R. Smith, and S. Ubol. 2013. Osteoclastogenesis induced by CHIKV-infected fibroblast-like synoviocytes: A possible interplay between synoviocytes and monocytes/macrophages in CHIKV-induced arthralgia/arthritis. Virus research. 177:179-188.
2012
Evanko, S.P., S. Potter-Perigo, P.L. Bollyky, G.T. Nepom, and T.N. Wight. 2012. Hyaluronan and versican in the control of human T-lymphocyte adhesion and migration. Matrix Biology. 31:90-100.
Hsu, J., Y. Gu, S.-L. Tan, S. Narula, J.A. DeMartino, and C. Liao. 2012. Bruton's Tyrosine Kinase mediates platelet receptor-induced generation of microparticles: A potential mechanism for amplification of inflammatory responses in rheumatoid arthritis synovial joints. Immunology letters, 150:97-104.
Rapko, S., and S. Duguay. 2012. Methods of evaluating cells and cell cultures. Patent Application US 20120329051 A1.
Tsuji, F., K. Oh-hashi, and K. Kiuchi. 2012. Differential effects of Akt pathway inhibitors on IL-1β-induced protein phosphorylation in human fibroblast-like synoviocytes. Journal of Receptors and Signal Transduction. 32:22-28.
Tsushima, H., K. Okazaki, M. Hayashida, T. Ushijima, and Y. Iwamoto. 2012. CCAAT/enhancer binding protein β regulates expression of matrix metalloproteinase-3 in arthritis. Annals of the rheumatic diseases. 71:99-107.
2011
Chen, D.P., C.K. Wong, L.S. Tam, E.K. Li, and C.W. Lam. 2011. Activation of human fibroblast-like synoviocytes by uric acid crystals in rheumatoid arthritis. Cellular & molecular immunology. 8:469-478.
Rapko, S., and S. Duguay. 2011. Methods of evaluating cells and cell cultures. Patent Application US 20120149013 A1.
Yamanaka, S., and K. Takahashi. 2011. Oct3/4, Klf4, c-Myc and Sox2 produce induced pluripotent stem cells. Patent US 8058065 B2.
2010
El-Beialy, W., N. Galal, Y. Deyama, Y. Yoshimura, K. Suzuki, K. Tei, and Y. Totsuka. 2010. Effects of Estrogen on PMCA 2 and 4 in Human Fibroblast-like Synovial Cells and Mouse Macrophage-like Cells. Endocrine J, 57:93-97.
Freimanis, G., P. Hooley, H.D. Ejtehadi, H. Ali, A. Veitch, P. Rylance, A. Alawi, J. Axford, A. Nevill, and P.G. Murray. 2010. A role for human endogenous retrovirusK (HML2) in rheumatoid arthritis: investigating mechanisms of pathogenesis. Clinical & Experimental Immunology. 160:340-347.
Rapko, S., M. Zhang, B. Richards, E. Hutto, S. Dethlefsen, and S. Duguay. 2010. Identification of the Chondrocyte Lineage Using Microfibril-Associated Glycoprotein-2, A Novel Marker That Distinguishes Chondrocytes from Synovial Cells. Tissue Engineering Part C: Methods. 16:1367-1375.
2009
Itoh, Y., N. Hatano, H. Hayashi, K. Onozaki, K. Miyazawa, and K. Muraki. 2009. An environmental sensor, TRPV4 is a novel regulator of intracellular Ca2+ in human synoviocytes. American Journal of Physiology. 297:C1082-C1090.
Nakamachi, Y., S. Kawano, M. Takenokuchi, K. Nishimura, Y. Sakai, T. Chin, R. Saura, M. Kurosaka, and S. Kumagai. 2009. MicroRNA124a is a key regulator of proliferation and monocyte chemoattractant protein 1 secretion in fibroblastlike synoviocytes from patients with rheumatoid arthritis. Arthritis & Rheumatism. 60:1294-1304.
Oki, K., F. Tsuji, K. Ohashi, M. Kageyama, H. Aono, and M. Sasano. 2009. The investigation of synovial genomic targets of bucillamine with microarray technique. Inflammation Research. 58:571-584.
2008
Galal, N., W.E. Beialy, Y. Deyama, Y. Yoshimura, T. Yoshikawa, K. Suzuki, and Y. Totsuka. 2008. Effect of estrogen on bone resorption and inflammation in the temporomandibular joint cellular elements. Int. J. Mol. medicine. 21:785.
Mathieu, M.C., S. LordDufour, V. Bernier, Y. Boie, J.D. Burch, P. Clark, D. Denis, Y. Han, J.R. Mortimer, and A.G. Therien. 2008. Mutual antagonistic relationship between prostaglandin E2 and IFNγ: Implications for rheumatoid arthritis. European journal of immunology. 38:1900-1912.
Pappas, J., and P. Yang. 2008. Human ESC vs. iPSC—Pros and Cons. J Cardiovascular Translational Res, 1:96-99.
Yamanaka, S., K. Takahashi, and M. Nakagawa. 2008. Nuclear reprogramming factor and induced pluripotent stem cells. Patent Application US 20090047263 A1.
2007
Carman, J., S. Nadler, M. Bowen, M. Neubauer, and P. Lu. 2007. Antiinflammatory agents; antiarthritic agents; determination gene expression of cell binding to drugs; calibration. Patent US 7244573 B2
Harigai, T., H. Hagiwara, Y. Ogawa, T. Ishizuka, S. Kaneda, and J. Kimura. 2007. Prednisolone phosphatecontaining TRX20 liposomes inhibit cytokine and chemokine production in human fibroblastlike synovial cells: a novel approach to rheumatoid arthritis therapy. Journal of pharmacy and pharmacology. 59:137-143.
Ikeda-Dantsuji, Y., I. Konomi, and A. Nagayama. 2007. Effects of levofloxacin and doxycycline on interleukin-6 production of Chlamydia trachomatis-infected human synovial fibroblasts. Chemotherapy. 53:332-337.
Rapko, S., U. Baron, U. Hoffmüller, F. Model, L. Wolfe, and S. Olek. 2007. DNA methylation analysis as novel tool for quality control in regenerative medicine. Tissue engineering. 13:2271-2280.
Takahashi, K., K. Tanabe, M. Ohnuki, M. Narita, T. Ichisaka, K. Tomoda, and S. Yamanaka. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131:861-872.
Tsuji, F., I. Seki, H. Aono, N. Odani, K. Mizutani, M. Okamoto, and M. Sasano. 2007. Bucillamine mechanism inhibiting IL-1β-induced VEGF production from fibroblast-like synoviocytes. International Immunopharmacol. 7:1569-1576.
2006
Wen, D., Y. Nong, J.G. Morgan, P. Gangurde, A. Bielecki, J. DaSilva, M. Keaveney, H. Cheng, C. Fraser, and L. Schopf. 2006. A selective small molecule IκB kinase β inhibitor blocks nuclear factor κB-mediated inflammatory responses in human fibroblast-like synoviocytes, chondrocytes, and mast cells. J. Pharmacol. & Experimental Therapeutics. 317:989-1001.
2005
Kunsch, C., J. Luchoomun, X.-l. Chen, G.L. Dodd, K.S. Karu, C.Q. Meng, E.M. Marino, L.K. Olliff, J.D. Piper, and F.-H. Qiu. 2005. AGIX-4207 [2-[4-[[1-[[3, 5-Bis (1, 1-dimethylethyl)-4-hydroxyphenyl] thio]-1-methylethyl] thio]-2, 6-bis (1, 1-dimethylethyl) phenoxy] acetic acid], a novel antioxidant and anti-inflammatory compound: cellular and biochemical characterization of antioxidant activity and inhibition of redox-sensitive inflammatory gene expression. J.Pharmacol.& Exp. Therap. 313:492-501.
Ota, T., T. Isogai, T. Nishikawa, K. Hayashi, K. Otsuka, J. Yamamoto, S. Ishii, T. Sugiyama, A. Wakamatsu, K. Nagai, T. Otsuki, S. Funahashi, S. Miyata, K. Sobue, and K. Hayashi. 2005. The protein encoded by novel gene has two WW domains that participate in protein interactions in the N-terminal domain, and this protein binds to other proteins, thus regulates the intracellular signal transduction, gene expression. Patent US 6908748 B2.
Wada, Y., K. Shimada, T. Kimura, and S. Ushiyama. 2005. Novel p38 MAP kinase inhibitor R-130823 suppresses IL-6, IL-8 and MMP-13 production in spheroid culture of human synovial sarcoma cell line SW 982. Imm. lett. 101:50-59.
2004
Patturajan, M., I. Millet, S. Kumar, C. Miller, P. Mezes, D. Lepley, C. Burgess, and R. Shimkets. 2004. Novel fibroblast growth factor and uses thereof. Patent Application US 20060141565 A1.
Tanaka, K., H. Moriaoka, T Morii, H. Matsumoto, L. Weisshbacc,, and F. Hornicek.  2004. DOWN REGULATION OF VEGF EXPRESSION IN HUMAN FIBROBLAST LIKE SYNOVIOCYTES BY NOVEL ANTI ANGIOGENIC PROTEIN PLASMINOGEN RELATED PROTEIN. 51st Annual Meeting of the Orthopaedic Research Society, Poster No: 1490. 
2002
Sugiyama, T., S. Ishii, J.-i. Yamamoto, R. Irie, K. Saito, T. Otuki, A. Wakamatsu, Y. Suzuki, Y. Hio, T. Ota, T. Nishikawa, S. Sugano, Y. Masuho, and T. Isogai. 2002. cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFα. FEBS letters. 517:121-128.
1999
Tsuji, F., K. Oki, T. Senda, M. Horiuchi, and S. Mita. 1999. Effects of mitogen-activated protein kinase inhibitors or phosphodiesterase inhibitors on interleukin-1-induced cytokines production in synovium-derived cells. Immunology letters. 68:275-279.

CCAAT/enhancer binding protein β regulates expression of matrix metalloproteinase-3 in arthritis.
Tsushima, H., Okazaki, K., Hayashida, M., Ushijima, T., Iwamoto, Y.
Ann. Rheum. Dis. 2012; 71:99–107.

Novel hyaluronic acid-methotrexate conjugates for osteoarthritis treatment.
Homma A, Sato H, Okamachi A, Emura T, Ishizawa T, Kato T, Matsuura T, Sato S, Tamura T, Higuchi Y, Watanabe T, Kitamura H, Asanuma K, Yamazaki T, Ikemi M, Kitagawa H, Morikawa T, Ikeya H, Maeda K, Takahashi K, Nohmi K, Izutani N, Kanda M, Suzuki R.
Bioorg Med Chem. 2009 Jul 1;17(13):4647-56. Epub 2009 May 3.

MicroRNA-124a is a key regulator of proliferation and monocyte chemoattractant protein 1 secretion in fibroblast-like synoviocytes from patients with rheumatoid arthritis.
Nakamachi Y, Kawano S, Takenokuchi M, Nishimura K, Sakai Y, Chin T, Saura R, Kurosaka M, Kumagai S.
Arthritis Rheum. 2009 May;60(5):1294-304.

**Mutual antagonistic relationship between prostaglandin E(2) and IFN-gamma: Implications for rheumatoid arthritis.
Mathieu MC, Lord-Dufour S, Bernier V, Boie Y, Burch JD, Clark P, Denis D, Han Y, Mortimer JR, Therien AG.
Eur J Immunol. 2008 Jul;38(7):1900-12.

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors.
K Takahashi, K Tanabe, M Ohnuki, M Narita, T Ichisaka, K Tomoda, S Yamanaka
Cell. 2007 Nov 30; 131(5): 861-872.

Effects of Levofloxacin and Doxycycline on Interleukin-6 Production of Chlamydia trachomatis-Infected Human Synovial Fibroblasts.
Y Ikeda-Dantsuji, I Konomi, A Nagayama
Chemotherapy. 2007;53:332-337.

TWEAK Is a Novel Arthritogenic Mediator.
SJ Perper, B Browning, LC Burkly, S Weng, C Gao, K Giza, L Su, L Tarilonte, T Crowell, L Rajman, L Runkel, M Scott, GJ Atkins, DM Findlay,  TS Zheng, H Hess
J Immunol. 2006 Aug 15;177(4):2610-20.

A Selective Small Molecule IKK-beta Inhibitor Blocks NF-kappa-B Mediated Inflammatory Responses in Human Fibroblast Like Synoviocytes, Chondrocytes and Mast Cells.
D Wen, Y Nong, JG Morgan, P Gangurde, A Bielecki, J DaSilva, M Keaveney, H Cheng, C Fraser, L Schopf, M
Hepperle, G Harriman, BD Jaffee, TD Ocain, Y Xu
J Pharmacol Exp Ther. 2006 Jun;317(3):989-1001.

PUMA regulation and proapoptotic effects in fibroblast-like synoviocytes.
HS Cha, S Rosengren, DL Boyle, GS Firestein
Arthritis Rheum. 2006 Feb;54(2):587-92.

Activation of synoviocytes by the secreted phospholipase A2 motif in the VP1-unique region of parvovirus B19 minor capsid protein.
J Lu, N Zhi, S Wong, KE Brown
J Infect Dis. 2006 Feb 15;193(4):582-90.

Novel p38 MAP kinase inhibitor R-130823 suppresses IL-6, IL-8 and MMP-13 production in spheroid culture of human synovial sarcoma cell line SW 982.
Y Wada, K Shimada, T Kimura, S Ushiyama
Immunology Letters. 2005 October 101(1):50-59.

AGIX-4207, a novel antioxidant and anti-inflammatory compound: I. Cellular and biochemical characterization of antioxidant activity and inhibition of redox-sensitive inflammatory gene expression.
C Kunsch, J Luchoomun, X Chen, G Dodd, K Karu, CQ Meng, EM Marino, LK Olliff, JD Piper, FH Qiu, JA Sikorski, PK Somers, KL Suen, S Thomas, AM Whalen, MA Wasserman, CL Sundell
J Pharmacol Exp Ther. 2005 May;313(2):492-501.

- See more at: http://www.cellapplications.com/cell-culture-citations#sthash.h1MUGlH8.dpuf



НазваниеКодЦена
HFLS Total Kit: Media, Subculture Reagents & Cells, Adult 408K-05a 104394.57 руб.



НазваниеКодЦена
Cryopreserved Ampoule 408-05a 86580.02 руб.



НазваниеКодЦена
Human FGF9 ELISA Kit CL0348 52944.28 руб.



НазваниеКодЦена
Total RNA prepared from Human Fibroblast-Like Synoviocytes, adult 408-R10a 40486.8 руб.
Total RNA prepared from Human Fibroblast-Like Synoviocytes, adult 408-R25a 80974.29 руб.



НазваниеКодЦена
Proliferating HFLS 409-6Wa 105764.92 руб.
Proliferating HFLS 409-96Wa 120713.75 руб.
Proliferating HFLS 409-25a 86580.02 руб.
Proliferating HFLS 409-75a 105764.92 руб.



НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.



НазваниеКодЦена
Basal medium (contains no growth supplement).В  Add GS before use. 414-500 6228.74 руб.



НазваниеКодЦена
All-in-one ready-to-use 415-500 11834.47 руб.
Growth medium without antibiotics 415A-500 12831.15 руб.
Growth medium without FBS 415F-500 12831.15 руб.



НазваниеКодЦена
Added to Basal Medium to create Growth Medium 415-GS 6602.41 руб.



Extended Family Products

НазваниеКодЦена
100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.



НазваниеКодЦена
Human Fibroblast Growth Factor acidic RP1029-10 9343.11 руб.
Human Fibroblast Growth Factor acidic RP1029-1000 180634.8 руб.
Human Fibroblast Growth Factor acidic RP1029-50 22423.6 руб.



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НазваниеКодЦена
Basal medium & growth supplement sold together packaged separately 415K-500 12831.15 руб.
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