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Клетки эндотелия аорты крысы: RAOEC. Первичные клеточные линии Cell Applications.

Rat Aortic Endothelial Cells: RAOEC

Клетки Эндотелия Аорты Крысы  (RAOEC) обеспечивают прекрасную модельную систему для изучения всех аспектов сердечно-сосудистых функций и заболеваний и были использованы в десятках научных публикаций. Положительный клеточный тест на DiI-Ac-LDL поглощение.

Параметры

Tissue:
Adult rat aorta.  Each lot is tested negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
3rd passage, >500,000 cells in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved  RAOEC (R304-05a), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100) and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Rat Endothelial Cell Growth Medium at 4th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least to 6th passage


Документы



Публикации

2016
Iba, T., T. Hamakubo, I. Nagaoka, K. Sato and T. Jecko. 2016. Physiological levels of pentraxin 3 and albumin attenuate vascular endothelial cell damage induced by histone H3 in vitro. Microcirculation, DOI: 10.1111/micc.12269.
Otto, S., A. Deussen, B. Zatschler, B. Muller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2016.  A novel role of endothelium in activation of latent pro-membrane type 1 MMP and pro-MMP-2 in rat aorta. Cardiovascular Research, 109:409-418.
2015
Ding, Q., Y. Hussain, J. Chorazyczewski, R. Gros, and R. Feldman. 2015. GPER-independent effects of estrogen in rat aortic vascular endothelial cells. Molecular and Cellular Endocrinology, 399:60-68.
DiVincenzo, L. 2015. DIRECTION OF INDUCED PLURIPOTENT STEM CELL DIFFERENTIATION BY ENDOTHELIAL CELL SECRETOME. Masters Thesis, Kent State University.
Iaconetti, C., S. De Rosa, A. Polimeni, S. Sorrentino, C. Gareri, A. Carino, J. Sabatino, A. Curcio, and C. Indolfi. 2015. Down-Regulation of miR-23b Induces Phenotypic Switching of Vascular Smooth Muscle Cells in vitro and in vivo. Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv141.
Otto, S., A. Deussen, B. Zatschler, B.Müller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2015. A Novel Role of Endothelium in Activation of Latent Pro-Membrane Type 1 Matrix Metalloproteinase and Pro-Matrix Metalloproteinase-2 in Rat Aorta.  Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv256.
Zhu, S., M. Zhang, J. Davis, W. Wu, K. Surrao, H. Wang and G. Wu. 2015. A single mutation in helix 8 enhances the angiotensin II type 1a receptor transport and signaling. Cellular Signalling, 27:2371-2379.
2014
Ahmed, S., N. Anzures, Z. Bosley, B. Bui, A. Feizi, S. Jawahery, C. Koenig, K. Lakomy, M. Lin, P. Natarajan, E. Nathan, H. Sayed, and E. Solano. 2014. THE APPLICATION OF AN EXOGENOUS LINEAR AND RADIAL ELECTRICAL FIELD TO AN IN VITRO CHRONIC DIABETIC ULCER MODEL FOR EVALUATION AS A POTENTIAL TREATMENT.  U Maryland, Digital Repository, http://hdl.handle.net/1903/15531.
Byun, E., N. Sung, M. Yang, D. Song, E. Byun, J. Kim, J. Park, B. Song, J. Lee, S. Park, M. Byun, and J. Kim. 2014. Procyanidin C1 Causes Vasorelaxation Through Activation of the Endothelial NO/cGMP Pathway in Thoracic Aortic Rings. Journal of Medicinal Food, 17:742-748.
Hamid, H., A. Azlina, M. Khamis, R. Rahman, N. Razak, S. Mutum, and A. Samsudin. 2014. Effect of Biphasic Calcium Phosphate Treated with Vascular Endothelial Growth Factor on Osteogenesis and Angiogenesis Gene Expression In Vitro. IFMBE Proceedings, 43:239-242.
Kopaliani, I. M. Martin, B. Zatschler, K. Bortlik, B. Müller, and A. Deussen. 2014. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Research in Cardiology, June, 109:419.
Mears, B., F. Shiao, N. Toney, C. Chen, K. Choi, C. Chow, M. Navin, and H. Shiau. 2014. Salvianolic Acid B Inhibits Growth of Cervical Cancer Cells in vitro via Induction of Apoptosis through the Extrinsic Pathway. U Maryland, Digital Repository.
2013
Gros, R., Q. Ding, B. Liu, J. Chorazyczewski, and R.D. Feldman. 2013. Aldosterone mediates its rapid effects in vascular endothelial cells through GPER activation. American Journal of Physiology-Cell Physiology. 304:C532-C540.
Nguyen, H., V.L. Chiasson, P. Chatterjee, S.E. Kopriva, K.J. Young, and B.M. Mitchell. 2013. Interleukin-17 causes Rho-kinase-mediated endothelial dysfunction and hypertension. Cardiovascular Research. 97:696-704.
Padilla, J., and N. Jenkins. 2013. Induction of endoplasmic reticulum stress impairs insulin-stimulated vasomotor relaxation in rat aortic rings: role of endothelin-1. J Physiol Pharmacol. 64:557-564.
2012
Byun, E., T. Ishikawa, A. Suyama, M. Kono, S. Nakashima, T. Kanda, T. Miyamoto, and T. Matsui. 2012. A procyanidin trimer, C1, promotes NO production in rat aortic endothelial cells via both hyperpolarization and PI3K/Akt pathways.  European Journal of Pharmacology, 692: Issues 1–3, 5 October:52–60.
Byun, E., S. Korematsu, T. Ishikawa, T. Nishizuka, S. Ohshima, T.l Kanda, and T. Matsui. 2012. Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels. J Nutional Biochem, 3:278-286.
Liu, B. 2012. The role of GRK2 in hypertension and regulation of GPR30. MS Thesis, University of Western Ontario.
Liu, X., Y. Cheng, J. Yang, L. Xu and C. Zhang. 2012. Cell-specific effects of miR-221/222 in vessels: molecular mechanism and therapeutic application. J Mol Cell Cardiol. 52:245-255. RAOSMC
Masuda, Y., Y. Yamada, and Y. Kimura. 2012. In Vitro Guidance of Dental Pulp Cells by Nd:YAG Laser-Irradiated Endothelial Cells. Photomed Laser Surg, 30:315-319.
2011
Aslam, M., D. Gündüz, D. Schuler, L. Li, F. Sharifpanah, D. Sedding, H.M. Piper, and T. Noll. 2011. Intermedin induces loss of coronary microvascular endothelial barrier via derangement of actin cytoskeleton. Cardiovascular research. 92:276-286.
Chiasson, V.L., M.A. Quinn, K.J. Young, and B.M. Mitchell. 2011. Protein Kinase CβII-Mediated Phosphorylation of Endothelial Nitric Oxide Synthase Threonine 495 Mediates the Endothelial Dysfunction Induced by FK506. J. Pharm.&Exp.Therap.337:718-723.
Duffy, G.P., T.M. McFadden, E.M. Byrne, S.-L. Gill, E. Farrell, and F.J. O’Brien. 2011. Towards in vitro vascularisation of collagen-GAG scaffolds. European cells & materials. 21:15-30.
2010
Ibrahim, S., Q. Kang, and A. Ramamurthi. 2010. The Impact of HA Oligomer Content on Physical, Mechanical, and Biologic Properties of Divinyl Sulfone-Crosslinked HA Hydrogels. J Biomed Mater Res A, 94:355-370.
Imamura, H., T. Ohta, K. Tsunetoshi, K. Doi, K. Nozaki, Y. Takagi, and K.-i. Kikuta. 2010. Transdifferentiation of bone marrow-derived endothelial progenitor cells into the smooth muscle cell lineage mediated by tansforming growth factor-β1. Atherosclerosis. 211:114-121.
Lionetti, V., S. Cantoni, C. Cavallini, F. Bianchi, S. Valente, I. Frascari, E. Olivi, G. Aquaro, F. Bonavita, I. Scarlata, M. Maioli, V. Vaccari, R. Tassinari, A. Bartoli, F. Recchia, G. Pasquinelli, and C. Ventura. 2010. Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation. JBC, 285:9949-9961.
2008
Hanley, E., J. Lauer, B. Micales, G. Lyons, and J. Shohet. 2008. Surface-directed differentiation of embryonic stem cells. Appl, Phys Lett, 92:193902.
2007
Mattagajasingh, I., C.-S. Kim, A. Naqvi, T. Yamamori, T.A. Hoffman, S.-B. Jung, J. DeRicco, K. Kasuno, and K. Irani. 2007. SIRT1 promotes endothelium-dependent vascular relaxation by activating endothelial nitric oxide synthase. Proceedings of the National Academy of Sciences. 104:14855-14860.
Ohkawara, N., H. Ueda, S. Shinozaki, T. Kitajima, Y. Ito, H. Asaoka, A. Kawakami, E. Kaneko, and K. Shimokada. 2007. Hepatocyte Growth Factor Fusion Protein Having Collagen-Binding Activity (CBD-HGF) Accelerates Re-endothelialization and Intimal Hyperplasia in Balloon-injured Rat Carotid Artery. J Atherosclerosis and Thrombosis, 14:185-191.
2001
Gousseva, N., K. Kugathasan, C.N. Chesterman, and L.M. Khachigian. 2001. Early growth response factor-1 mediates insulin-inducible vascular endothelial cell proliferation and regrowth after injury*. Journal of cellular biochemistry. 81:523-534.
 


НазваниеКодЦена
For general cryopreservation of most primary cells. Contains FBS & DMSO. 040-50 6228.74 руб.



НазваниеКодЦена
Cryopreserved RAOEC R304-05a 95300.12 руб.



НазваниеКодЦена
RAOEC Total Kit: Media, Subculture Reagents & Cells, Adult R304K-05a 116104.7 руб.



НазваниеКодЦена
Cultured and shipped at fourth passage in Rat Endothelial Cell Growth Medium. R305-25a 95300.12 руб.
Proliferating RAOEC R305-75a 118969.73 руб.
Proliferating RAOEC R305-6Wa 118969.73 руб.
Proliferating RAOEC R305-96Wa 128935.85 руб.



НазваниеКодЦена
Total RNA prepared from Rat Aortic Endothelial Cells R304-R10a 52944.28 руб.
Total RNA prepared from Rat Aortic Endothelial Cells R304-R25a 105889.25 руб.



НазваниеКодЦена
Added to Basal Medium to create Growth Medium R211-GS 6851.75 руб.



НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.



Extended Family Products

НазваниеКодЦена
AFS 123-100 2242.7 руб.



НазваниеКодЦена
100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.



НазваниеКодЦена
25 x 24-Well Rxns TF101KS 6222.62 руб.
250 x 24-Well Rxns TF101K 49829.91 руб.



Related Products

НазваниеКодЦена
Basal medium (contains no growth supplement).В  Add GS before use. R210-500 6851.75 руб.



НазваниеКодЦена
All-in-one ready-to-use R211-500 12706.82 руб.



НазваниеКодЦена
Basal medium & growth supplement sold together packaged separately R211K-500 13703.5 руб.
Growth medium kit without phenol red R211KPR-500 15696.86 руб.




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