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Клетки гладкой мускулатуры аорты крысы: RAOSMC. Первичные клеточные линии Cell Applications.

Rat Aortic Smooth Muscle Cells: RAOSMC

Клетки Гладкой Мускулатуры Аорты Крысы (RAOSMC) обеспечивают прекрасную модельную систему для изучения всех аспектов сердечно-сосудистых функций и заболеваний, особенно связанных с механизмами гиперплазии и гипертрофии клеток гладкой мускулатуры интимы, ведущих к закупорке сосудов при атеросклерозе и рестенозе стента.

RAOSMC от Cell Applications, Inc. были использованы в десятках научных исследований:

  • Обеспечили «золотой стандарт» контроля для маркеров экспрессии в гладкой мускулатуре α-SMA, кальпонина и кальдесмона.
  • Прояснили сигнальные пути цитокинов и факторов роста, задействованных в молекулярной регуляции деления, миграции гладкомышечных клеток и общих сосудистых функциях.
  • Продемонстрировано, что экстракт персика ингибирует внутриклеточный Ca2+ и активные формы кислорода, показывая возможность для предотвращения сердечно-сосудистых заболеваний.
  •  Изучение свойств внеклеточного матрикса и его роли в здоровье сердечно-сосудистой системы, восстановления поврежденных сосудов и успешной инженерии тканей.
  • Продемонстрировано, что продукты деградации эластина, индуцированные гипергликемией, ведут к кальцификации гладкомышечных клеток сосудов.
  • Идентифицирован периостин, также задействованный при механическом стрессе и воспалительном ответе, как ранний маркер кальцификации гладкомышечных клеток.
  • Разработана передовая технология стентирования, включая новейшие материалы поверхности и системы доставки препаратов для предотвращения рестеноза и других окклюзивных сосудистых патологий.

Характеристика: положительно для специфичной для клеток гладкой мускулатуры альфа-актиновой экспрессии

НазваниеКодЦена
Cryopreserved RAOSMC R354-05a 39630.21 руб. 1 Ampoule



НазваниеКодЦена
RAOSMC Total Kit: Media, Subcultre Reagents & Cells R354K-05a 53784.13 руб. 1 Kit



НазваниеКодЦена
Proliferating RAOSMC R355-25a 39630.21 руб. T-25 Flask
Proliferating RAOSMC R355-6Wa 53784.13 руб. 6 well
Proliferating RAOSMC R355-75 по запросу T-75 Flask
Proliferating RAOSMC R355-96W по запросу 96 well
Proliferating RAOSMC R355-6WВ по запросу 6 well
Proliferating RAOSMC R355-25 по запросу T-25 Flask
Proliferating RAOSMC R355-96Wa 62276.23 руб. 96 well







Extended Family Products

НазваниеКодЦена
100 tests 028-S 3963.02 руб. Sample
500 tests 028-01 15337.25 руб. 1 bottle



НазваниеКодЦена
125 x 24-Well TF350K 31506.4 руб. 1 Kit
25 x 24-Well Rxns TF350KS 5938.7 руб. 1 Sample Kit



НазваниеКодЦена
Total RNA Prepared from Rat Aortic Smooth Muscle Cells R354-R25a 77279.55 руб. 25 ug
Total RNA prepared from Rat Aortic Smooth Muscle Cells R354-R10a 38639.45 руб. 10 ug



Related Products

НазваниеКодЦена
Basal medium (contains no growth supplement).В  Add GS before use. R310-500 6657.77 руб. 500 ml



НазваниеКодЦена
All-in-one ready-to-use R311-500 12127.03 руб. 500 ml



НазваниеКодЦена
Basal medium & growth supplement sold together packaged separately R311K-500 13078.23 руб. Yields 500 ml



НазваниеКодЦена
Added to Basal Medium to create Growth Medium R311-GS 6539.11 руб. 50 ml



НазваниеКодЦена
Provides signals that change cell behavior R311I-250 7490.32 руб. 250 ml



НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6063.19 руб. 100 ml



Параметры

Tissue:
Tunica intima and media of healthy adult rat aorta. Each lot is tested negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
2nd passage, >500,000 cells in Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved  RAOSMC (R354-05a), 500 ml of Rat SMC Growth Medium (R311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Rat Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Документы

Публикации


2016
Otto, S., A. Deussen, B. Zatschler, B. Muller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2016.  A novel role of endothelium in activation of latent pro-membrane type 1 MMP and pro-MMP-2 in rat aorta. Cardiovascular Research, 109:409-418.
Pugh, R., J. Slee, S. Farwell, Y. Li, T. Barthol, W. Patton and L. Lowe-Krentz. 2016. Transmembrane protein 184A is a receptor required for vascular smooth muscle cell responses to heparin. JBC, doi: 10.1074/jbc.M115.681122.
2015
Chuang, T., W. Pearce, and O. Khorram. 2015. miR-29c Induction Contributes to Down-Regulation of Vascular Extracellular Matrix Proteins by Glucocorticoids. Am J Physiol, DOI: 10.1152/ajpcell.00254.2014
Otto, S., A. Deussen, B. Zatschler, B. Müller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2015. A Novel Role of Endothelium in Activation of Latent Pro-Membrane Type 1 Matrix Metalloproteinase and Pro-Matrix Metalloproteinase-2 in Rat Aorta.  Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv256.
Ueda, K., K. Yoshimura, O. Yamashita, T. Harada, N. Morikage, and K. Hamano. 2015. Possible Dual Role of Decorin in Abdominal Aortic Aneurysm. PLoS ONE 10: e0120689.
Zhu, S., M. Zhang, J. Davis, W. Wu, K. Surrao, H. Wang, and G. Wu. 2015. A single mutation in helix 8 enhances the angiotensin II type 1a receptor transport and signaling.  Cellular Signalling, 27:2371-2379.
2014
Kopaliani, I. M. Martin, B. Zatschler, K. Bortlik, B. Müller, and A. Deussen. 2014. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Research in Cardiology, June, 109:419.
2013
Kono, R., Y. Okuno, M. Nakamura, K.-i. Inada, A. Tokuda, M. Yamashita, R. Hidaka, and H. Utsunomiya. 2013. Peach (Prunus persica) extract inhibits angiotensin II-induced signal transduction in vascular smooth muscle cells. Food chemistry. 139:371-376.
Li, Y., R. Bhindi, Z.J. Deng, S.W. Morton, P.T. Hammond, and L.M. Khachigian. 2013. Inhibition of vein graft stenosis with a c-jun targeting DNAzyme in a cationic liposomal formulation containing 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)/1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). International Journal of Cardiology. 168:3659-3664.
Shanmugam, I., A. Sinha, R. Norris, R. Markwald, and N. Vyavahare. 2013. Periostin as an early marker for elastin mediated vascular smooth muscle cell calcification. Cardiovascular System. 1.
Sinha, A., and N.R. Vyavahare. 2013. High-glucose levels and elastin degradation products accelerate osteogenesis in vascular smooth muscle cells. Diabetes & vascular disease research : official journal of the International Society of Diabetes and Vascular Disease. 10:410-419.
Yamashita, O., K. Yoshimura, A. Nagasawa, K. Ueda, N. Morikage, Y. Ikeda, and K. Hamano. 2013. Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm. PloS one. 8:e79753.
Yamauchi, H., N. Motomura, U.-i. Chung, M. Sata, D. Takai, A. Saito, M. Ono, and S. Takamoto. 2013. Growth-associated hyperphosphatemia in young recipients accelerates aortic allograft calcification in a rat model. The Journal of Thoracic and Cardiovascular Surgery. 145:522-530.
2012
Ko, W.-c., B.-c. Chen, M.-j. Hsu, C.-t. Tsai, C.-y. Hong, and C.-h. Lin. 2012. Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway. Acta pharmacologica Sinica. 33:49-56.
Malabanan, K.P., A.V. Sheahan, and L.M. Khachigian. 2012. Platelet-Derived Growth Factor-BB Mediates Cell Migration through Induction of Activating Transcription Factor 4 and Tenascin-C. The American journal of pathology. 180:2590-2597.
Midwinter, R.G., G.J. Maghzal, J.M. Dennis, B.J. Wu, H. Cai, A.A. Kapralov, N.A. Belikova, Y.Y. Tyurina, L.-F. Dong, L. Khachigian, J. Neuzil, V.E. Kagan, and R. Stocker. 2012. Succinobucol induces apoptosis in vascular smooth muscle cells. Free Radical Biology and Medicine. 52:871-879.
Minami, T., K. Kuwahara, Y. Nakagawa, M. Takaoka, H. Kinoshita, K. Nakao, Y. Kuwabara, Y. Yamada, C. Yamada, J. Shibata, S. Usami, S. Yasuno, T. Nishikimi, K. Ueshima, M. Sata, H. Nakano, T. Seno, Y. Kawahito, K. Sobue, A. Kimura, R. Nagai, and K. Nakao. 2012. Reciprocal expression of MRTFA and myocardin is crucial for pathological vascular remodelling in mice. EMBO J., 31:4428-4440.
Santiago, F.S. 2012. Regulatory Mechanisms in Vascular Injury and Repair. University of New South Wales, PhD dissertation.
2011
Ko, W. 2011. Connective Tissue Growth Factor and Cardiovascular DiseaseFocusing on the Mechanisms of Structural Remodeling in Atrial Fibrillation and Atherothrombosis. Dissertation, Graduate Institute of Clinical Medicine, U0007-1801201207171100.
Taite, L.J., and J.L. West. 2011. Sustained delivery of nitric oxide from poly (ethylene glycol) hydrogels enhances endothelialization in a rat carotid balloon injury model. Cardiovascular Engineering and Technology. 2:113-123.
2010
Cheluvaraju, C. 2010. Characterization of anti-proteolytic and anti-proliferative activities of pentagalloylglucose; its potential application as a therapeutic agent in vascular diseases. Clemson University, PhD Dissertation.
Imamura, H., T. Ohta, K. Tsunetoshi, K. Doi, K. Nozaki, Y. Takagi, and K.-i. Kikuta. 2010. Transdifferentiation of bone marrow-derived endothelial progenitor cells into the smooth muscle cell lineage mediated by transforming growth factor-β1. Atherosclerosis. 211:114-121.
Ni, J., A. Waldman, and L. Khachigian. 2010. c-Jun Regulates Shear- and Injury-inducible Egr-1 Expression, Vein Graft Stenosis after Autologous End-to-Side Transplantation in Rabbits, and Intimal Hyperplasia in Human Saphenous Veins. JBC, 285:4038-4048.
Pappan, K. 2010. Hmg-coa secondary metabolites and uses thereof. Patent Application US 20120121554 A1.
2009
Adams, J.W., J. Ramirez, Y. Shi, W. Thomsen, J. Frazer, M. Morgan, J.E. Edwards, W. Chen, B.R. Teegarden, and Y. Xiong. 2009. APD791, 3-methoxy-n-(3-(1-methyl-1h-pyrazol-5-yl)-4-(2-morpholinoethoxy) phenyl) benzamide, a novel 5-hydroxytryptamine 2A receptor antagonist: pharmacological profile, pharmacokinetics, platelet activity and vascular biology. Journal of Pharmacology and Experimental Therapeutics. 331:96-103.
Alexis, J., N. Wang, W. Che, N. Lerner-Marmarosh, A. Sahni, V. Korshunov, Y. Zou, B. Ding, C. Yan, B. Berk, and J. Abe. 2009. Bcr Kinase Activation by Angiotensin II Inhibits Peroxisome Proliferator-Activated Receptor γ Transcriptional Activity in Vascular Smooth Muscle Cells. Circulation Research, 104:67-78.
Cooper, K., I. Chun, D., Colter, D., Dhanaraj, S., Gosiewska, A., Seyda, A. Fang, C. and Ynag, C. Tissue engineered blood vessels. Patent US 20090275129 A1.
Guha, S. 2009. The Role of GSK-3 in Notch Signalling in Vascular Smooth Muscle Cells. PhD Dissertation, Dublin City University.
Khachigian, L. 2009. Antiproliferative agents; administering protein; restenosis, atherosclerosis; cancer. Patent US 7541343 B2.
Kothapalli, C.R., and A. Ramamurthi. 2009. Copper nanoparticle cues for biomimetic cellular assembly of crosslinked elastin fibers. Acta Biomaterialia. 5:541-553.
Kothapalli, C.R., C.E. Gacchina, and A. Ramamurthi. 2009. Utility of hyaluronan oligomers and transforming growth factor-beta1 factors for elastic matrix regeneration by aneurysmal rat aortic smooth muscle cells. Tissue engineering. Part A. 15:3247-3260.
Kothapalli, C.R., P.M. Taylor, R.T. Smolenski, M.H. Yacoub, and A. Ramamurthi. 2009. Transforming growth factor beta 1 and hyaluronan oligomers synergistically enhance elastin matrix regeneration by vascular smooth muscle cells. Tissue engineering. Part A. 15:501-511.
Liu, M.Y., and L.M. Khachigian. 2009. HDAC-1 is Enriched at the PDGF-D Promoter in Response to IL-1beta and Forms a Cytokine-Inducible Gene Silencing Complex with NF-kappaB p65 and IRF-1. Journal of Biological Chemistry:10.1074/jbc.M1109.061903.
McSpadden, L.C., R.D. Kirkton, and N. Bursac. 2009. Electrotonic loading of anisotropic cardiac monolayers by unexcitable cells depends on connexin type and expression level. American Journal of Physiology-Cell Physiology. 297:C339-C351.
Morrow, D., J.P. Cullen, W. Liu, S. Guha, C. Sweeney, Y.A. Birney, N. Collins, D. Walls, E.M. Redmond, and P.A. Cahill. 2009. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol. 29:1112-1118.
2008
Adams, J.W., J. Ramirez, D. Ortuno, Y. Shi, W. Thomsen, J.G. Richman, M. Morgan, P. Dosa, B.R. Teegarden, H. Al-Shamma, D.P. Behan, and D.T. Connolly. 2008. Anti-thrombotic and vascular effects of AR246686, a novel 5-HT2A receptor antagonist. European journal of pharmacology. 586:234-243.
Atkins, D., A. Baker, L. Khachigian. 2008. Catalytic molecules. Patent US 7355035 B1.
Frew, R., Y. Wang, T.M. Weiss, P. Nelson, and T.W. Sawyer. 2008. Attenuation of maitotoxin-induced cytotoxicity in rat aortic smooth muscle cells by inhibitors of Na+/Ca2+ exchange, and calpain activation. Toxicon. 51:1400-1408.
Malabanan, K.P. 2008. Roles of Activation Transcription Factor 4 (ATF4) and YrdC in the Response of Vascular Smooth Muscle Cells to Injury. In Centre for Vascular Research, School of Medical Sciences. University of New South Wales, Australia, PhD dissertation.
Poletek, P. 2008. Signalwege der C-Peptid-induzierten Proliferation glatter Gefäßmuskelzellen. Dissertation, Universität Ulm.
Shi, F., and J. Sottile. 2008. Caveolin-1-dependent beta1 integrin endocytosis is a critical regulator of fibronectin turnover. Journal of cell science. 121:2360-2371.
Taite, L.J., P. Yang, H.-W. Jun, and J.L. West. 2008. Nitric oxide-releasing polyurethane–PEG copolymer containing the YIGSR peptide promotes endothelialization with decreased platelet adhesion. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 84B:108-116.
2007
Csiszar, A., N. Labinskyy, K. Smith, A. Rivera, Z. Orosz, and Z. Ungvari. 2007. Vasculoprotective Effects of Anti-Tumor Necrosis Factor-α Treatment in Aging. Am J Pathol, 1770:388-398.
2006
Csiszar, A., M. Ahmad, K.E. Smith, N. Labinskyy, Q. Gao, G. Kaley, J.G. Edwards, M.S. Wolin, and Z. Ungvari. 2006. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype. The American journal of pathology. 168:629-638.
Joddar, B., and A. Ramamurthi. 2006. Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells. Biomaterials. 27:5698-5707.
Joddar, B., and A. Ramamurthi. 2006. Fragment size- and dose-specific effects of hyaluronan on matrix synthesis by vascular smooth muscle cells. Biomaterials. 27:2994-3004.
Liu, M.Y., M. Eyries, C. Zhang, F.S. Santiago, and L.M. Khachigian. 2006. Inducible platelet-derived growth factor D-chain expression by angiotensin II and hydrogen peroxide involves transcriptional regulation by Ets-1 and Sp1. Blood. 107:2322-2329.
Nakane, M., T. Kolasa, R. Chang, L. Miller, R. Moreland, and J. Brioni. 2006. Acrylamide Analog as a Novel Nitric Oxide-Independent Soluble Guanylyl Cyclase Activator. J Pharmacol Sci, 102:231-238.
Ross, J.J., Z. Hong, B. Willenbring, L. Zeng, B. Isenberg, E.H. Lee, M. Reyes, S.A. Keirstead, E.K. Weir, and R.T. Tranquillo. 2006. Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells. Journal of Clinical Investigation. 116:3139-3149.
Taite, L.J., and J.L. West. 2006. Poly(ethylene glycol)-lysine dendrimers for targeted delivery of nitric oxide. Journal of Biomaterials Science, Polymer Edition. 17:1159-1172.
West, S., E. Ogru, and R. Gianello. 2006. Compounds having anti-cancer properties. Patent Application US 20090233881 A1.
2005
Campagnolo, L., A. Leahy, S. Chitnis, S. Koschnick, M. Fitch, J. Fallon, D. Loskutoff, M. Taubman, and H. Stuhlmann. 2005. EGFL7 Is a Chemoattractant for Endothelial Cells and Is Up-Regulated in Angiogenesis and Arterial Injury. Am J Pathol, 167:275-284.
Morrow, D., C. Sweeney, Y.A. Birney, P.M. Cummins, D. Walls, E.M. Redmond, and P.A. Cahill. 2005. Cyclic strain inhibits Notch receptor signaling in vascular smooth muscle cells in vitro. Circulation research. 96:567-575.
Pintucci, G., P.-J. Yu, F. Saponara, D.L. Kadian-Dodov, A.C. Galloway, and P. Mignatti. 2005. PDGF-BB induces vascular smooth muscle cell expression of high molecular weight FGF-2, which accumulates in the nucleus. Journal of cellular biochemistry. 95:1292-1300.
Sato, Y., R. Nakamura, M. Satoh, K. Fujishita, S. Mori, S. Ishida, T. Yamaguchi, K. Inoue, T. Nagao, and Y. Ohno. 2005. Thyroid Hormone Targets Matrix Gla Protein Gene Associated With Vascular Smooth Muscle Calcification. Circulation research. 97:550-557.
Sottile, J., and J. Chandler. 2005. Fibronectin Matrix Turnover Occurs through a Caveolin-1–dependent Process. Molecular biology of the cell. 16:757-768.
2004
Deng, Y.-M., B.J. Wu, P.K. Witting, and R. Stocker. 2004. Probucol Protects Against Smooth Muscle Cell Proliferation by Upregulating Heme Oxygenase-1. Circulation. 110:1855-1860.
Santiago, F.S., and L.M. Khachigian. 2004. Ets-1 stimulates platelet-derived growth factor A-chain gene transcription and vascular smooth muscle cell growth via cooperative interactions with Sp1. Circulation research. 95:479-487.
West, S. and Ogru, E. 2004. Compounds having anti-proliferative properties. Patent Application US 20060241085 A1.
2002
Khachigian, L. 2002. Treatment of cancer. Patent Application US 20030203864 A1.
Khan, K.F., D.J. Falcone, and R. Kraemer. 2002. Nerve growth factor activation of Erk-1 and Erk-2 induces matrix metalloproteinase-9 expression in vascular smooth muscle cells. Journal of Biological Chemistry. 277:2353-2359.
2001
Andrade-Gordon, P., C.K. Derian, B.E. Maryanoff, H.-C. Zhang, M.F. Addo, W.-m. Cheung, B.P. Damiano, M.R. D'Andrea, A.L. Darrow, and L. de Garavilla. 2001. Administration of a potent antagonist of protease-activated receptor-1 (PAR-1) attenuates vascular restenosis following balloon angioplasty in rats. Journal of Pharmacology and Experimental Therapeutics. 298:34-42.
Santiago, F.S., H.C. Lowe, Y.V. Bobryshev, and L.M. Khachigian. 2001. Induction of the Transcriptional Repressor Yin Yang-1 by Vascular Cell Injury: AUTOCRINE/PARACRINE ROLE OF ENDOGENOUS FIBROBLAST GROWTH FACTOR-2. Journal of Biological Chemistry. 276:41143-41149.
1999
Andrade-Gordon, P., B.E. Maryanoff, C.K. Derian, H.-C. Zhang, M.F. Addo, A.L. Darrow, A.J. Eckardt, W.J. Hoekstra, D.F. McComsey, D. Oksenberg, E.E. Reynolds, R.J. Santulli, R.M. Scarborough, C.E. Smith, and K.B. White. 1999. Design, synthesis, and biological characterization of a peptide-mimetic antagonist for a tethered-ligand receptor. Proceedings of the National Academy of Sciences. 96:12257-12262.
Day, F., L. Rafty, C. Chesterman, and L. Khachigian. 1999. Angiotensin II (ATII)-inducible Platelet-derived Growth Factor A-chain Gene Expression Is p42/44 Extracellular Signal-regulated Kinase-1/2 and Egr-1-dependent and Mediated via the ATII Type 1 but Not Type 2 Receptor. J Biol Chem, 274:23726-23733.
Santiago, F., D. Atkins, and nL. Khachigian. 1999. Vascular Smooth Muscle Cell Proliferation and Regrowth after Mechanical Injury in Vitro Are. Egr-1/NGFI-A-Dependent. Am J Pathol, 155:897-905.

Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой