BACKGROUND Dosage compensation is an essential regulatory process in organisms whose primary sex-determining mechanisms cause males and females to differ in their relative dose of sex chromosomes. By equalizing sex-linked gene expression, dosage compensation mechanisms prevent the sex-specific lethality that arises as a consequence of a twofold difference in sex chromosome dosage.1
DPY-30 is a novel nuclear protein of 123 amino acids. It is an essential component of dosage compensation machinery that reduces X chromosome transcript levels in hermaphrodites (XX). Loss of DPY-30 activity results in XX-specific lethality.2
DPY-30 is required for the sex-specific association of DPY-27 (a chromosome condensation protein homolog) with the hermaphrodite X chromosomes. DPY-30 itself is not associated with the X chromosomes, nor is its pattern of expression perturbed by mutations in the gene hierarchy that controls dosage compensation. Therefore, DPY-30 is a ubiquitous factor that is likely to promote the hermaphrodite-specific association of DPY-27 with the X chromosome by affecting the activity of a sex-specific dosage compensation gene.3 In XO animals, DPY-30 is required for developmental processes other than dosage compensation, including: coordinated movement, normal body size, correct tail morphology and mating behavior.
In yeast, the homolog of DPY-30, Saf19p, functions as a member of histone 3 lysine 4 methylation complex, which is the key part of epigenetic developmental control.4 The human DPY-30 is a conserved member of in specific histone methyltransferase (HMT) complexes. In the human MLL1 (mixed-lineage leukemia-1) HMT complex, DPY-30 binds to the BRE2 homolog ASH2L in order to regulate histone 3–lysine 4 trimethylation.
1. Meyer BJ: WormBook, 25:1-14, 2005.
2. Hsu DR & Meyer BJ: Genetics, 137:999-1018, 1994.
3. Hsu DR et al.: Development, 121:3323-34,1995.
4. Nagy PL et al.: Proc Natl Acad Sci U S A, 99:90-4, 2002.
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Human DPY-30 C-terminal protein sequence.
Species & predicted
Weight of protein:
Detects endogenous levels of DPY-30 protein in various cell lysates.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.