BACKGROUND Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It induces programmed cell death, or ‘apoptosis’, in cells expressing its cognate receptor, Fas (CD95/APO-1) that belongs to the subgroup of the tumor necrosis factor receptor (TNF-R) family containing an intracellular death domain (DD). Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer.1 Fas forms the death inducing signalling complex (DISC) upon ligand binding.2 Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. FADD also contains a death effector domain (DED) near its amino terminus, which facilitates binding to the DED of FADD-like ICE (FLICE), more commonly referred to as caspase-8.3 FLICE can then self-activate through proteolytic cleavage into p10 and p18 subunits, two each of which form the active heterotetramer enzyme. Active caspase-8 is then released from the DISC into the cytosol, where it cleaves other effector caspases, eventually leading to DNA degradation, membrane blebbing, and other hallmarks of apoptosis.4
1. Strasser A et al.: Immunity, 30:180-192, 2009.
2. Mundle SD & Raza A: Immunol. Today, 23: 187-194, 2002.
3. Qiu J et al.: J. Neurosci. 22:3504-3511, 2002.
4. Tsuyuki et al.: J Clin Invest., 96: 2924–2931, 1995.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Peptide containing human FAS-L C-terminal sequence.
Species & predicted
reactivity ( ):
Human, Rat, Mouse
IHC (Paraffin) 1:100
Weight of protein:
Detects endogenous levels of FAS-L protein in normal primary cell lysates.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.