Anti-GFP: Mouse GFP Antibody |
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BACKGROUND Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).
REFERENCES
1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol7, 557–62.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
| Cat.No.: | CC10047 |
| Antigen: | GFP fusion protein |
| Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | All |
Applications & Suggested starting dilutions:* | WB 1:1000 IP 1:50 IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 27 kDa |
| Specificity/Sensitivity: | Detects endogenous GFP proteins without cross-reactivity with family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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