BACKGROUND Nuclear hormone receptors are ligand-dependent transcription factors that require coactivators to regulate target gene expression. The steroid receptor coactivator-3 (SRC-3), also known as p/CIP, RAC3, AIB1, ACTR and TRAM-1, is a cancer-amplified coactivator in the SRC gene family which also includes SRC-1 and SRC-2. SRC-3 interacts with nuclear receptors and a subset of transcription factors, recruits histone acetyltransferases and methyltransferases for chromatin remodeling, and facilitates target gene transcription.1 SRC-3 phosphorylation and methylation have been shown to regulate coactivator complex assembly.2,3 Accumulated results from both ex vivo and animal model studies indicate that SRC-3 plays important roles in many biological processes involving cell proliferation, cell migration, cell differentiation, somatic growth, sexual maturation, female reproductive function, and vasoprotection.1 Moreover, SRC-3 has been associated with multiple cancers, including breast, gastric and prostate cancers.4 The phosphorylation of Thr24 in SRC-3 can be mediated by activation of MAP kinase pathway, which may regulate function and localization of SRC-3 proteins in cells.5
1. Liao, L. et al.: J Steriod Biochem Mol Biol 83:3, 2002.
2. Feng, Q. et al.: Mol Cell Biol 26:7846, 2006.
3. Wu, H. et al.: J Biol Chem 281:21848, 2006.
4. Yan, J. et al.: Acta Pharmacol Sin 27:387, 2006.
5. Wu, R.C. et al.: Mol. Cell 5:937-49, 2004.
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Sequence surrounding and including Thr24 of human SRC-3/AIB-1.
Affinity Purified Rabbit IgG
Species & predicted
reactivity ( ):
IHC (Paraffin) n/d
Weight of protein:
Detects endogenous phospho-human and mouse SRC-3/AIB-1.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.