BACKGROUND Spleen tyrosine kinase (Syk) is a non-receptor protein tyrosine kinase expressed in a wide range of hematopoietic cells.1 Syk has not only been recognized as a key player in both innate and adaptive immunity, but there is also evidence of a role for Syk in non-immune cells and in the maintenance of vascular integrity,2 as well as the pathogenesis of malignant cancer.3 Syk contains two SH2 domains in tandem, and multiple auto-phosphorylation sites. Syk is activated upon binding of tandem SH2 domains to the immuno-receptor tyrosine-based activating motif (ITAM) of various types of receptors such as Fc-gamm-R, CR3, Dectin-1, and apoptotic cell-recognizing receptor. Syk is critical for the tyrosine phosphorylation of multiple proteins which regulate important pathways downstream of the receptor, such as Ca2+ mobilization, mitogen-activated protein kinase (MAPK) cascades, PI-3 kinase pathway and many other signaling pathways.4
1. Nilsson, S. et al: Physiol Rev 81:1535–65, 2001.
2. Tremblay, G. B. et al: Mol Endocrinol. 11: 353-365, 1997.
3. Mathews, J & Gustafsson, J. A.: Mol. Interv. 3:281-92, 2003.
4. Tremblay, A. et al: Mol Cell 3, 513–519, 1999.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Sequence surrounding and including Tyr525 and Tyr526 of human Syk.
Affinity purified rabbit IgG
Species & predicted
reactivity ( ):
Human, Mouse, Rat
IHC (Paraffin) n/d
Weight of protein:
Detects endogenous phospho-human, mouse and rat Syk proteins.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.