Anti-Zap70: Monoclonal Zap70 Antibody |
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BACKGROUND ZAP-70 is a protein tyrosine kinase (PTK) required for T-cell development and T-cell antigen receptor (TCR) function. ZAP-70 is associated with the phosphorylated antigen receptor and undergoes tyrosine phosphorylation following receptor activation. It was demonstrated that tyrosine phosphorylation of ZAP-70 resulted in an increase in its catalytic activity and that this activation is mediated by the phosphorylation of tyrosine residue 493 by the Src family of PTKs.1 Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the Zap-70 gene resulting in the absence of ZAP-70 expression. More recently, it has been shown that deregulation of ZAP-70 activity can induce autoimmune diseases. Finally, ZAP-70 expression has been shown in some B chronic lymphocytic leukemia and correlated with bad prognosis of the disease. The diversity of pathologies associated with deregulation of ZAP-70 demonstrates its key role in immune responses. Research aiming at deciphering the different signaling pathways regulated by ZAP-70 will not only shed some lights on these pathologies, but will also help finding new pharmacological tools targeting ZAP-70, to induce immunosuppression or tolerance.2
REFERENCES
1. Chan, AC et al. : EMBO J. 14:2499, 1995.
2. Hivroz, C., Med Sci (Paris). 21:150, 2005.
2. Hivroz, C., Med Sci (Paris). 21:150, 2005.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CB10249 |
Antigen: | Recombinant human Zap70 protein |
Isotype: | Mouse Monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 70 kDa |
Specificity/Sensitivity: | Reacts specifically with human ZAP70 protein in Western Blot applications. |
Storage: | Store at 4° for frequent use; at -20° for at least one year |
*Optimal working dilutions must be determined by end user.
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