Anti-Zap70: Mouse Zap70 Antibody |
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BACKGROUND The Syk family protein tyrosine kinase Zap70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap70 kinase activity and downstream signaling events. Expression of Zap70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (CLL) (7, 8).
REFERENCES
1. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167–180.
2. Iwashima, M. et al. (1994) Science 263, 1136–1139.
3. Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171–3178.
4. Chan, A.C. et al. (1995) EMBO J. 14, 2499–2508.
5. Williams, B.L. et al. (1999) EMBO J. 18, 1832–1844.
6. Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285–6294.
7. Wiestner, A. et al. (2003) Blood 101, 4944–4951.
8. Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764–1775.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
| Cat.No.: | CC10056 |
| Antigen: | Recombinant human Zap70 expressed in E. Coli |
| Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:100 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 70 kDa |
| Specificity/Sensitivity: | Detects endogenous Zap70 proteins without cross-reactivity with other family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
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