BACKGROUND The renin-angiotensin-aldosterone system regulates renal vasomotor activity, maintains optimal salt and water homeostasis, and controls tissue growth in the kidney. Renin produced from the juxtaglomerular apparatus of the kindney, splits angiotensinogen to produce the inactive decapeptide angiotensin I. The latter is then converted to the powerful octapeptide vasoconstrictor, angiotensin II by the action of angiotensin converting enzyme (ACE). ACE also inactivates the vasodilating nonapeptide bradykinin, which theoretically contributes to the hypertensive effects of ACE activity. Additionally, It has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.1
Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and plays a critical role in blood pressure regulation. The ACE precursor consists of 1306-amono acid residues including a 29 amino acid residue-signal peptide. The mature protein has a molecular weight of 180kDa. There are two isoforms of ACE, a smaller/ single catalytic site enzyme found in the testes and a more widely expressed ~180kDa dual active site isoform. ACE is often membrane bound.2 ACE inhibitors are widely used to treat hypertension and cardiovascular diseases. ACE inhibitors have also been used to slow nephropathy, particularly when it is associated with diabetes.3 The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120 kDa.
1. Regoli, D. et al: Pharmacol Rev. 26:69-123, 1974
2. Kessler, S. P. et al: J. Biol. Chem. 275:26259-64, 2000
3. Cody, R.J.: Drugs 47:586-98, 1994
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Target Protein Species:
156 pg/ml – 10000pg/ml
No detectable cross-reactivity with other cytokines
Store at 4°C. Use within 6 months.
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.