BACKGROUND: Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect™ Skeletal Muscle Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of smooth muscle cells. This kit contains Cytofect-2 and Media for transfection and culturing cells. The result has been high transfection efficiency and viability for these traditionally hard-to-transfect primary smooth muscle cells.
Cytofect™ Skeletal Muscle Cell Transfection Kit contains all the necessary reagents and media for transfection. Optimized transfection protocols are provided with the kit to carry out successful transfection of the following smooth muscle cells with high efficiency (15-30%) and viability (80%-95%):
Products are for research use only. They are not intended for human, animal, or diagnostic applications. CF1 is manufactured by Targeting Systems.
Antibiotics-Free Growth Medium
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.