BACKGROUND Chemokines (chemotactic cytokines) belong to a family of chemoattractant molecules involved in the directed migration of immune cells. Over fifty human chemokines have been identified that can be categorised into four groups; CC, CXC, CX3C and C (XCL1 and XCL2); depending on the spacing of their first two cysteine residues. Chemokines exert their effects by binding to G-protein-coupled chemokine receptors on the surface of cells, predominantly leukocytes. Eighteen human chemokine receptors have been identified that are classified according to the class of chemokines that they bind.1 The major function of chemokines is to regulate leukocyte trafficking in hematopoiesis and in innate and adaptive immunity. Other functions include angiogenic activity, apoptosis, T-cell differentiation and phagocyte activation. Inadvertent activation of chemokine receptors leads to autoimmunity by inappropriately targeting self antigens for destruction by cytotoxic T-cells and macrophages.2
CCR2 is a receptor for the MCP-1, MCP-3 and MCP-4 chemokines, which specifically mediate monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. CCR2 transduces a signal by increasing the intracellular calcium ions level and also mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase.3 It may be alternative coreceptor with CD4 for HIV-1 infection.4 Its encoding gene is located in the chemokine receptor gene cluster region. CCR2 has two isoforms.
1. Zlotnik, A. & Yoshie, O.:Immunity 12:121-27, 2000
2. Locati, M.: Ann. Rev. Med. 50:425-40, 1999
3. Salcedo,R. et al: Blood 96:34-40, 2000
4. Kostrikis, L.G. et al: Nature Med. 4:350-3, 1998
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
|Synthetic peptide corresponding to a region within amino acids 1 and 69 of CKR-2B according to NP_000638|
Species & predicted
reactivity ( ):
Weight of protein:
|Detects endogenous CCR2 proteins without cross-reactivity with other family members.|
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.