BACKGROUND CD40L (also called CD154) is a member of the tumor necrosis factor family of ligands, plays a major role in immune responses via its receptor, CD40.1 CD40L is mainly expressed on activated CD4+ T lymphocytes. Recently, CD40L has been detected on the surfaces of activated platelets and shown to activate endothelium. It was also shown that HUVEC cell express CD40L.2 Whereas CD40, a member of the tumor necrosis factor receptor (TNFR) family, has been found on the surface of B lymphocytes, monocytes, hematopoietic progenitors, dendritic cells (DCs), endothelial cells, epithelial cells and so on. CD40L-CD40 interaction plays important role in control of thymus-dependent humoral immunity and cell mediated immune responses. CD40L is the most crucial molecule for activating antigen-presenting cells (APCs) and thereby initiating the immune response.3 Deletion or mutations of the CD40L molecule leads to severe immunodeficiency, in both man and mouse, characterized by hypogamma-globulinemia as well as T cell defects in cell mediated immunity.4 Moreover, blockade of the CD40L/CD40 pathway by antibodies to CD40L has been used, in experimental animals,5 to effectively prevent and treat a variety of autoimmune diseases as well as allograft rejection without inducing generalized immune suppression.
1. Daoussis D et al.: Clin. Diagn. Lab. Immunol. 11:635-641, 2004.
2. Mendez-Cruz AR et al.: Immunol. Lett. 111:116-123, 2007.
3. Boisvert J et al.: J. Immunol. 173: 3647-3652, 2004.
4. Weller S et al.: Proc. Natl. Acad. Sci. USA 98:1166-1170, 2001.
5. Lutgens E et al.: Proc. Natl. Acad. Sci. USA 97:7564-7469, 2000.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Human CD40L carboxyl-terminal sequence
Species & predicted
reactivity ( ):
Human, Rat, Mouse
IHC (Paraffin) 1:100
Weight of protein:
Detects endogenous levels of CD40L protein in normal primary cell lysates.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.