Centromere-associated protein E (CENP-E) is a protein that in humans is encoded by the CENP-E gene.1 It is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle, first appearing at the centromere region of chromosomes during prometaphase, and is rapidly degraded upon completion of mitosis.
Molecular characterization of the CENP-E molecule shows it to have a tri-partite structure comprised of amino and carboxy-terminal globular domains separated by a ~1,500 residue α-helical domain predicted to form coiled-coils. The amino-terminal domain contains striking homology to the microtubule-dependent motor protein kinesin, thus demonstrating CENP-E to be a member of the growing family of kinesin-like proteins.2
CENP-E was reported to be associated with a minus end microtubule motor activity and to provide functions that are critical for normal chromosome motility during mitosis. In vitro studies showed that it is responsible for microtubule depolymerization-dependent movement of chromosomes.3 Instead of utilizing ATP, chromosome movement in this in vitro system relies simply on the ability of the kinetochore to remain attached to the shrinking end of a single microtubule induced to depolymerize by the dilution of free tubulin subunits. CENP-E was also found to regulate recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network through its association with STX4 and SNAP25. Recent studies showed that CENP-E has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis.
1. Testa, J. R. et al: Genomics. 23(3):691-3, 1995.
2. Goldstein, L. S. B. Annu. Rev. Genetics. 27:319-351, 1993.
3. Lombillo, V. A. et al: J. Cell Biol. 128:107-115, 1995.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Short peptide from human CENP-E.
Species & predicted
reactivity ( ):
WB 1:500 - 1:1000
Weight of protein:
Detects endogenous CENP-E proteins without cross-reactivity with other family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.