BACKGROUND The insulin receptor substrate (IRS) proteins are cytoplasmic docking proteins that function as essential signaling intermediates downstream of activated cell surface receptors. The IRS proteins do not contain intrinsic kinase activity but rather function by organizing signaling complexes to initiate intracellular signaling cascades. The insulin receptor substrate-2 (IRS-2) is a protein homologous to IRS-1, originally identified as a protein phosphorylated in response to interleukin-4.1 It is a major insulin signalling molecule. The initial mechanism of insulin action involves its binding to specific cell surface receptors leading to the autophosphorylation and activation of an intrinsic tyrosine kinase associated with the beta-receptor subunit. IRS proteins (IRS-1 and IRS-2) are substrates for the insulin receptor and other tyrosine kinases associated with the receptors of growth factors and cytokines.2 IRS proteins act as an interface between activated receptors and signaling proteins with Src homology 2 (SH2) domains. After insulin stimulation, IRS-1 and IRS-2 associate with several proteins including phosphatidylinositol (PI) 3- kinase, Syp, Nck, Grb2, and Fyn,3 which mediate the downstream insulin signaling. IRS-2 inactivation in mice induces a form of diabetes characterized by peripheral insulin resistance and reduced beta cell mass.
1. Wang, L. M. et al ：EMBO J. 11：4899，1992.
2. Dearth, R. K. et al: Cell Cycle. 6:705,2007.
3. Wick, K. R. et al: J. Biol. Chem. 278:8460, 2003.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
KLH-conjugated peptide containing human IRS-2 sequence close to its C-terminal end
Species & predicted
reactivity ( ):
Human, Mouse, Rat
Weight of protein:
Detects endogenous levels of IRS-2 proteins. Does not cross-react with other IRS family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.