BACKGROUND Spleen tyrosine kinase (Syk) is a non-receptor protein tyrosine kinase expressed in a wide range of hematopoietic cells.1 Syk has not only been recognized as a key player in both innate and adaptive immunity, but there is also evidence of a role for Syk in non-immune cells and in the maintenance of vascular integrity,2 as well as the pathogenesis of malignant cancer.3 Syk contains two SH2 domains in tandem, and multiple auto-phosphorylation sites. Syk is activated upon binding of tandem SH2 domains to the immuno-receptor tyrosine-based activating motif (ITAM) of various types of receptors such as Fc-gamm-R, CR3, Dectin-1, and apoptotic cell-recognizing receptor. Syk is critical for the tyrosine phosphorylation of multiple proteins which regulate important pathways downstream of the receptor, such as Ca2+ mobilization, mitogen-activated protein kinase (MAPK) cascades, PI-3 kinase pathway and many other signaling pathways.4
1. Nilsson, S. et al: Physiol Rev 81:1535–65, 2001.
2. Tremblay, G. B. et al: Mol Endocrinol. 11: 353-365, 1997.
3. Mathews, J & Gustafsson, J. A.: Mol. Interv. 3:281-92, 2003.
4. Tremblay, A. et al: Mol Cell 3, 513–519, 1999.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Purified recombinant human Syk fragments expressed in E. coli.
Species & predicted
reactivity ( ):
Weight of protein:
Detects endogenous Syk proteins without cross-reactivity with other family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.