BACKGROUND Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis.1 The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.2 The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa.3 Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.4 Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.5 The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.6 The standard product used in this kit is FAS extracellular sections (1-173aa), in addition to the soluble human IgG1 Fc domain. The molecular mass is 45KDa.
1.Dhein, J, et al: Nature 373: 438-441, 1995.
2.Itoh, N.; et al: Cell 66: 233-243, 1991.
3.Oehm, A.; et al: J. Biol. Chem. 267: 10709-10715, 1992.
4.Arscott, P. L. et al: J. Clin. Endocr. Metab. 84: 4246-4252, 1999.
5.Desbarats, J.; et al: Nature Cell Biol. 5: 118-125, 2003.
6.Zhang, X. et al: J. Med. Genet. 42: 479-484, 2005.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Target Protein Species:
31.2 pg/ml – 2000 pg/ml
No detectable cross-reactivity
with other cytokines
Store at 4°C. Use within 6 months.
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.