IL-17F: Rat Interleukin-17F |
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BACKGROUND Interleukin 17F (IL-17F) is one of six members of the IL-17 family (IL-17A-F) secreted by activated CD4+ T cells and monocytes. Similar to IL-17A, IL-17F binds to the IL17 RC receptor and promotes the production of IL-6, IL-8, G-CSF and increases matrix turnover rates. IL-17F is also thought to inhibit angiogenesis and induce endothelial cells to produce IL-2, MCP-1 and TGF-β1.
Recombinant rat IL-17F is a non-glycosylated, disulfide-linked homodimer, containing a total of two 133 amino acids and having a total molecular mass of 30 kDa
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat. No.:
RP3028
Alternative Name:
None
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant rat IL-17F is lyophilized with no additives.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts and is typically 10-20 ng/mL.
AA Sequence:
MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWD YNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSV PIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHH AA
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
