IL-27/p28: Mouse Interleukin-27 (p28 subunit) |
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BACKGROUND The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines.
Параметры
Cat. No.:
RP2050
Alternative Name:
IL-30, P28, IL-27 p28 subunit
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant mouse IL-27/p28 is lyophilized at a concentration of 1 mg/ml in 10 mM sodium phosphophate buffer, pH 7.5.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is measured via dose-dependent inhibition of IL-17A expression from mouse CD4+ splenocytes stimulated with TGF-β and IL-6. 50ng/ml typically inhibits greater than 25% of IL-17A expression.
Документы
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