BACKGROUND Interleukin-31 (IL-31) is an immunoregulatory cytokine that is mainly expressed by activated TH2 cells. IL-31 signals through the IL-31 Receptor (IL-31RA) and the oncostain receptor (OSMR), which are expressed on monocytes, epithelial cells, and keratinocytes. IL-31 has a role in inflammatory skin diseases.
Recombinant mouse IL-31 is a non-glycosylated protein, containing 141 amino acids, with a mass of 15.7 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Sterile Filtered white lyophilized (freeze-dried) powder.
Recombinant mouse IL-31 is lyophilized at a concentration of 1 mg/ml in 10 mM sodium phosphophate buffer, pH 7.5.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
A standard activity has not yet been determined.
MTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C