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Porcine Aortic Endothelial Cells: PAOEC



Porcine Aortic Endothelial Cells: PAOEC

Porcine Aortic Endothelial Cells (PAOEC) provide a useful model system to study many aspects of cardiovascular function and disease.  Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.
PAOEC from Cell Applications, Inc., and they have been utilized in a number of research publications, including those demonstrating that:
  • H2O2 increases production of O2- and decreases ACh-induced NO accumulation, contributing to vascular dysfunction
  • Cell survival under oxidative stress depends on PKC-dependent phosphorylation of VEGFR-3 and its downstream signaling molecules Shc, GFRBP2, os, p85, SHP-2 and PLC
  • Replication of classical swine fever virus causes ERK and JNK mediated reduction in connexin 43 expression, and downregulates expression of NO synthase via ERK and PI3/Akt pathways
  • Direct interactions between endothelial cells and T cells trigger release of HMGB1, a proinflammatory molecule, implementing HMGB1 in graft rejection
  • The tetraspanin CD82 is the recognition sensor responsible for rejection of xenotransplants
  • Endothelial cells from heart valves align perpendicular to flow, while aortic endothelial cells align parallel to flow, indicating the need to match the cell types when designing engineered tissue devices
PAOEC have also been used to:
  • Investigate changes in expression of NO synthase, VCAM-1, c-jun, MCP-1 and ICAM-1 change in endothelial cells subjected to shear stress of varying magnitude and gradient
  • Develop methods to improve swine-to-human xenotransplantation by pretreating donors with 1-deamino-8-d-arginine vasopressin (DDAVP) to reduce vWF
  • Design endothelial implants to increase lumen diameter and replace heart valves

Параметры


Porcine Aortic Endothelial Cells: PAOEC

Tissue
Normal healthy porcine aorta
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 PAOEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PAOEC, Growth Medium (P211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 16


Документы


Porcine Aortic Endothelial Cells: PAOEC



Публикации


Porcine Aortic Endothelial Cells: PAOEC

2016
Ameer, G., M. Kibbe and A. Webb. 2016. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20160199535 A1.
Dushpanova, A., S. Agostini, E. Ciofini, M. Cabiati, V. Casieri, M. Matteucci, S. Del Ray, A. Clerico, S. Berti and V. Lionetti. 2016. Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells. Scientific Reports, 6:30048.
Grossini, E., S. Farruggio, F. Qoqaiche, G. Raina, L. Camillo, L. sigaudo, D. Mary, N. Surico and D. Surico. 2016. Monomeric adiponectin modulates nitric oxide release and calcium movements in porcine aortic endothelial cells in normal/high glucose conditions. Life Sciences, 161:1-9.
2015
Grossini, E., P. Marotta, S. Farruggio, L. Sigaudo, F. Qoqaiche, G. Raina, V. Giuli, D. Mary, G. VAcca, and F. Pollastro. 2015. Effects of Artemetin on Nitric Oxide Release and Protection against Peroxidative Injuries in Porcine Coronary Artery Endothelial Cells. Phytotherapy Research, doi: 10.1002/ptr.5386.
2014
Sierad, L., E. Shaw, R. Launius, S. McBride, C. Storholt, R. Poole, D. Spence, K. Miller, L. Sosdian, K. Allen, L. Burton, A. Iari, J. Nagatomi, and D. Simionesuc. 2014. Toward an Endothelial-Cell Covered Mechanical Valve; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves. Challenges in Regenerative Medicine, 1: Aug.
2013
Saleh, S.M., R.S. Parhar, R.S. Al-Hejailan, R.H. Bakheet, H.S. Khaleel, H.G. Khalak, A.S. Halees, M.Z. Zaidi, B.F. Meyer, and G.P. Yung. 2013. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation. J. of Immunology. 191:2796-2805.
Saleh, S.M., R.S. Parhar, R.S. Al-Hejailan, R.H. Bakheet, H.S. Khaleel, H.G. Khalak, A.S. Halees, M.Z. Zaidi, B.F. Meyer, G.P. Yung, J.D. Seebach, W. Conca, K.S. Khabar, K.S. Collison, and F.A. Al-Mohanna. 2013. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation. The Journal of Immunology. 191:2796-2805.
2011
Wang, C.-Y., H.-I. Yeh, T.-J. Chang, H.-J. Hsiao, M.-S. Tsai, S.-M. Tsai, and P.-A. Liu. 2011. Attenuation of nitric oxide bioavailability in porcine aortic endothelial cells by classical swine fever virus. Archives of virology. 156:1151-1160.
2010
Hsiao, H.-J., P.-A. Liu, H.-I. Yeh, and C.-Y. Wang. 2010. Classical swine fever virus down-regulates endothelial connexin 43 gap junctions. Archives of virology. 155:1107-1116.
Sierad, L., A. Simionescu, C. Albers, J. Chen, J. Maivelett, M. Tedder, J. Liao, and D. Simionescu. 2010. Design and Testing of a Pulsatile Conditioning System for Dynamic Endothelialization of Polyphenol-Stabilized Tissue Engineered Heart Valves. Cardiovascular Engineering and Technology. 1:138-153.
2009
Ameer, G., M. Kibbe, and A. Webb. 2009. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20100036476 A1.
2008
Allen, J., S. Khan, M.C. Serrano, and G. Ameer. 2008. Characterization of porcine circulating progenitor cells: toward a functional endothelium. Tissue Engineering Part A. 14:183-194.
Kim, Y.T., H.J. Lee, S.W. Lee, J.Y. Kim, H.C. Wi, S.J. Park, S.J. Bae, and H.J. Kang. 2008. Pre-treatment of porcine pulmonary xenograft with desmopressin: a novel strategy to attenuate platelet activation and systemic intravascular coagulation in an ex-vivo model of swine-to-human pulmonary xenotransplantation. Xenotransplantation. 15:27-35.
Meyer, R.D., D.B. Sacks, and N. Rahimi. 2008. IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. PloS one. 3:e3848.
Witting, P.K., B.S. Rayner, B.-J. Wu, N.A. Ellis, and R. Stocker. 2008. Hydrogen peroxide promotes endothelial dysfunction by stimulating multiple sources of superoxide anion radical production and decreasing nitric oxide bioavailability. Cellular Physiology and Biochemistry. 20:255-268.
2007
Kawahara, K., K. Setoyama, K. Kikuchi, K. Biswas, R. Kamimura, M. Iwata, T. Ito, Y. Morimoto, T. Hashiguchi, S. Takao, and I. Maruyama. 2007. HMGB1 release in co-cultures of porcine endothelial and human T cells. Xenotransplantation, 14:636-641.
2006
Zhang, W.-Y., E. Schwartz, Y. Wang, J. Attrep, Z. Li, and P. Reaven. 2006. Elevated Concentrations of Nonesterified Fatty Acids Increase Monocyte Expression of CD11b and Adhesion to Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology. 26:514-519.
2004
Wang, J., X. Zhang, and J. Groopman. 2004. Activation of Vascular Endothelial Growth Factor Receptor-3 and Its Downstream Signaling Promote Cell Survival under Oxidative Stress. J Biol Chem 279:27088-27097.
2002
Butcher, J. and R. Nerem. 2002. Morphological differences between aortic and aortic valve endothelial cells in static and fluid flow conditions. Engineering in Medicine and Biology, Biomedical Engineering Society EMBS/BMES Conference. 318-319, DOI10.1109/IEMBS.2002.1134512


Cryopreserved PAOEC
НазваниеКодЦена
Frozen PAOEC P304-05 72876.52 руб.



Cryopreserved PAOEC Total Kit
НазваниеКодЦена
Medium, Subculture Reagents & Cells P304K-05 90192.39 руб.



Proliferating PAOEC
НазваниеКодЦена
Actively growing, dividing cells in medium P305-25 72876.52 руб.
Actively growing, dividing cells in medium P305-75 96546.14 руб.
Actively growing, dividing cells in medium P305-6W 96546.14 руб.
Actively growing, dividing cells in medium P305-96W 111494.98 руб.



Extended Family Products

Cell Freezing Medium
НазваниеКодЦена
For general cryopreservation of most primary cells. Contains FBS & DMSO. 040-50 6228.74 руб.



Cyto-X Colorimetric Cell Counting Reagent
НазваниеКодЦена
100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.



Cytofect™ Endothelial Cell Transfection Kit
НазваниеКодЦена
25 x 24-Well Rxns TF101KS 6222.62 руб.
250 x 24-Well Rxns TF101K 49829.91 руб.



PAOEC RNA
НазваниеКодЦена
Total RNA prepared from Porcine Aortic Endothelial Cells P304-R10 40486.8 руб.
Total RNA prepared from Porcine Aortic Endothelial Cells P304-R25 80974.29 руб.



Subculture Reagent Kit
НазваниеКодЦена
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.



Related Products

Porcine Endothelial Cell Basal Medium
НазваниеКодЦена
Basal medium (contains no growth supplement). Add GS before use. P210-500 6287.85 руб.



Porcine Endothelial Cell Growth Medium
НазваниеКодЦена
All-in-one ready-to-use P211-500 12208.14 руб.



Porcine Endothelial Cell Growth Medium Kit
НазваниеКодЦена
Basal medium & growth supplement sold together packaged separately P211K-500 13080.49 руб.



Porcine Endothelial Cell Growth Supplement
НазваниеКодЦена
Added to Basal Medium to create Growth Medium P211-GS 6602.41 руб.




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