EMEM культуральная среда - набор
250 мл среды, базовой
12.5 ml hPL supplement (5% final), deep-frozen
EMEM (MEM Eagle) cell culture medium supplemented with hPL (human Platelate lysate)
The EMEM medium is one of the first chemically defined cell culture media developed in 1959 by H. Eagle. This medium is ideally suited for culturing a variety of normal and neoplastic cells and cell lines. For a couple of cell lines, the addition of NEAA (Non-Essential Amino Acids) and Pyruvate is recommended.
The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Contrary to FBS, hPL is a bystander product. Platelet preparations must be destroyed 5 days after collection from the donors unless they are used up in other applications. Only platelets from double-negative tested donors are used to manufacture the platelet lysates and are pooled from 25 donors.
EMEM supplemented with hPL delivers sufficient nutrients and growth factors to sustain the proliferation of cell lines such as MCF-7 breast cancer cells and Caco-2 colon carcinoma cells.
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