Культуральная среда Hams 12, набор
250 мл среды, базовой
12.5 ml hPL supplement (5% final), deep-frozen
Ham’s F12 cell culture medium supplemented with hPL (human Platelate lysate)
Ham’s F12 cell culture medium was formulated to support the serum free culture of CHO cells. It is also suitable for the culture of carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, as well as rat, rabbit, and chicken embryos.
The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Contrary to FBS, hPL is a bystander product. Platelet preparations must be destroyed 5 days after collection from the donors unless they are used up in other applications. Only platelets from double-negative tested donors are used to manufacture the platelet lysates and are pooled from 25 donors.
Ham’s F12 supplemented with hPL delivers sufficient nutrients and growth factors to sustain the proliferation of cell lines such as HepG2 hepatoma cells.
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