BseA I

Recognition Sequence:

Heat inactivation: No.

Ligation and recutting: After 40-fold overdigestion with BseA I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation:
Inhibition (Blocked): CpG
Inhibition (Blocked by overlapping): dam
Inhibition (Impaired by overlapping): dcm

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (24 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.