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Sal I 5' - G↓TCGA C - 3'
3' - C AGCT↑G - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Sal I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 2 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Sal I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 2 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-130L | 15849.17 руб. | 5 x 2000 Units | |
| S pack | EN-130S | 3962.51 руб. | 2000 Units |
| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
