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Taq I 5' - T↓CG A - 3'
3' - A GC↑T - 5' |
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Recognition Sequence:
5
Heat inactivation: 20 minutes at 80°C.
Ligation and recutting: After ten-fold overdigestion with Taq I, >90 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Incubation without BSA results in 50 % activity.
DNA Methylation:
No Inhibition: dcm, CpG
Inhibition (Blocked by overlapping): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 121 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Heat inactivation: 20 minutes at 80°C.
Ligation and recutting: After ten-fold overdigestion with Taq I, >90 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Incubation without BSA results in 50 % activity.
DNA Methylation:
No Inhibition: dcm, CpG
Inhibition (Blocked by overlapping): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 121 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
| Название | Код | Цена | Количество | |
| L pack | EN-142L | 15849.17 руб. | 5 x 3500 Units | |
| S pack | EN-142S | 3962.51 руб. | 3500 Units | |
| Тестовая позиция | PCR-OOOO | по запросу |
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| Документы |
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 Datasheet
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
