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Фибробласты сердца человека: HCF. Первичные клеточные линии Cell Applications.

Human Cardiac Fibroblasts: HCF

Фибробласты Сердца Человека (HDF) являются превалирующим видом клеток в сердце, составляя до 60-70 % всех клеток. HCF от Cell Applications, Inc. обеспечивают прекрасную модельную систему для изучения многих аспектов функций и патологий человеческого сердца.

HCF от Cell Applications, Inc. были использованы во множестве исследований, например:

(Текст на языке оригинала)
  • Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists
  • Show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites
  • Demonstrate that in response to mechanical stretch, cardiac fibroblasts release TGF-β which causes trombomodulin downregulation, increasing the risk of thromboembolic events and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP
  • Indicate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype
  • Identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology
  • Investigate the roles of scleraxis and AMPKα1 in scar formation following myocardial infarction
  • Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance
  • Report that express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels


Normal human heart tissue.  Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HCF (306-05f), 500 ml of Cardiac Fibroblast Growth Medium (316-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 8 doublings



Bagchi, R.., R. Wang, F. Jahan, J. Wigle and M. Czubryt. 2016. Regulation of scleraxis transcriptional activity by serine phosphorylation. J Molec & Cell Cardiol, doi:10.1016/j.yjmcc.2016.02.013.
Nagpal, V., R. Rai, A. Place, S. Murphy, S. Verma, A. Ghosh and D. Vaughan. 2016. MiR-125b Is Critical for Fibroblast-to-Myofibroblast Transition and Cardiac Fibrosis. Circulation, 133:291-301.
Momtahan, N., N. Poornejad, J. Struk, A. Castleton, B. Herrod, B. Vance, J. Eatough, D. Roeder, P. Reynolds, and D. Cook. 2015. Automation of Pressure Control Improves Whole Porcine Heart Decellularization. Tissue Engineering Part C: Methods, doi:10.1089/ten.TEC.2014.0709.
Philip, J., A. Razzaque, M. Han, J. Li, T. Thecanat, X. Xu, and S. Akhter. 2015. Regulation of Mitochondrial Oxidative Stress by β-arrestins in Cardiac Fibroblasts.  Disease Models & Mechanisms. doi: 10.1242/dmm.019968.
Tomita, K., M. Takashina, N. Mizuno, K. Sakata, K. Hattori, J. Imura, W. Ohashi, and Y. Hattori. 2015. Cardiac fibroblasts: contributory role in septic cardiac dysfunction. Journal of Surgical Research, 193:874–887.
Ichiki, T., J. Schirger, B. Huntley, F. Brozovich, J. Maleszewski, S. Sandberg, S. Sangaralingham, S. Park, and J. Burnett. 2014. Cardiac fibrosis in end-stage human heart failure and the cardiac natriuretic peptide guanylyl cyclase system: Regulation and therapeutic implications. J Molecular and Cellular Cardiol, 75:199-205.
Li, J., J. Philip, X. Xu, T. Theccanat, M. Razzaque, and S. Akhter. 2014. β-Arrestins regulate human cardiac fibroblast transformation and collagen synthesis in adverse ventricular remodeling. JMCC, 76:73-83.
Noppe, G., C. Dufeys, P. Buchlin, N. Marquet, D. Castanares-Zapatero, M. Balteau, N. Hermida, C. Bouzin, H. Esfahani, B. Viollet, L. Bertrand, J.-L. Balligand, J.-L. Vanoverschelde, C. Beauloye, and S. Horman. 2014. Reduced scar maturation and contractility lead to exaggerated left ventricular dilation after myocardial infarction in mice lacking AMPKα1. Journal of Molecular and Cellular Cardiology. 74:32-43.
Yue, Z., Y. Zhang, J. Xie, J. Jiang, and L. Yue. 2013. Transient Receptor Potential (TRP) channels and cardiac fibrosis. Current topics in medicinal chemistry. 13:270-282
Bagchi, R.A., and M.P. Czubryt. 2012. Synergistic roles of scleraxis and Smads in the regulation of collagen 1α2 gene expression. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1823:1936-1944.
Czubryt, M. 2012. Inhibition of collagen synthesis. Patent Application US 20140288003 A1.
Savvatis, K., S. van Linthout, K. Miteva, K. Pappritz, D. Westermann, J.C. Schefold, G. Fusch, A. Weithäuser, U. Rauch, P.-M. Becher, K. Klingel, J. Ringe, A. Kurtz, H.-P. Schultheiss, and C. Tschöpe. 2012. Mesenchymal Stromal Cells but Not Cardiac Fibroblasts Exert Beneficial Systemic Immunomodulatory Effects in Experimental Myocarditis. PloS one. 7:e41047.
Bogaard, H.J., S. Mizuno, A.A.A. Hussaini, S. Toldo, A. Abbate, D. Kraskauskas, M. Kasper, R. Natarajan, and N.F. Voelkel. 2011. Suppression of Histone Deacetylases Worsens Right Ventricular Dysfunction after Pulmonary Artery Banding in Rats. American journal of respiratory and critical care medicine. 183:1402-1410.
Chen, I. 2011. Use of kmup-3 for myocardial infarction. Patent Application US 20120095013 A1.
Czubryt, M. 2011. Modulation of scleraxis using a dominant negative scleraxis mutant with a basic dna-binding domain deletion. Patent Application US 20130123198 A1.
D'Souza, K.M., R. Malhotra, J.L. Philip, M.L. Staron, T. Theccanat, V. Jeevanandam, and S.A. Akhter. 2011. G Protein-coupled Receptor Kinase-2 Is a Novel Regulator of Collagen Synthesis in Adult Human Cardiac Fibroblasts. Journal of Biological Chemistry. 286:15507-15516.
Liu, C.P., J.L. Yeh, B.N. Wu, C.Y. Chai, I.J. Chen, and W.T. Lai. 2011. KMUP3 attenuates ventricular remodelling after myocardial infarction through eNOS enhancement and restoration of MMP9/TIMP1 balance. Brit. J. Pharmacol. 162:126-135.
Ma, X. 2011. Studies on signals mediating or preventing the intracrine induction of chromatin compaction and cell death by high molecular weight fibroblast growth factor 2. University of Manitoba, PhD dissertation.
Miteva, K., M. Haag, J. Peng, K. Savvatis, P.M. Becher, M. Seifert, K. Warstat, D. Westermann, J. Ringe, M. Sittinger, H.-P. Schultheiss, C. Tschöpe, and S. Van Linthout. 2011. Human Cardiac-Derived Adherent Proliferating Cells Reduce Murine Acute Coxsackievirus B3-Induced Myocarditis. PloS one. 6:e28513.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Methods of screening using a natriuretic peptide receptor. Patent US 7943296 B2.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Natriuretic peptide related fragment in cardiovascular disease. Patent Application US 20110218230 A1.
Peng, H., O.A. Carretero, E.L. Peterson, and N.-E. Rhaleb. 2010. Ac-SDKP inhibits transforming growth factor-β1-induced differentiation of human cardiac fibroblasts into myofibroblasts. American J. of Physiology 298:H1357-H1364.
Wang, Y.-J., M.-W. Lin, S.-N. Wu, and R.J. Sung. 2007. The activation by estrogen receptor agonists of the BKCa-channel in human cardiac fibroblasts. Biochemical pharmacology. 73:1347-1357. Young, E.F., and E.E. Marcantonio. 2007. A Novel Subcellular Collagen Organization Process Visualized by Total Internal Reflection Fluorescence Microscopy. Cell Communication and Adhesion. 14:169-180.
Wang, Y.-J., R. Sung, M.-W. Lin, and S.-N. Wu. 2006. Contribution of BKCa-Channel Activity in Human Cardiac Fibroblasts to Electrical Coupling of Cardiomyocytes-Fibroblasts. J Membrane Biol. 213:175-185.
Dubey, R.K., E.K. Jackson, D.G. Gillespie, M. Rosselli, F. Barchiesi, A. Krust, H. Keller, L.C. Zacharia, and B. Imthurn. 2005. Cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites mediate estradiol-induced antimitogenesis in human cardiac fibroblast. Journal of Clinical Endocrinology & Metabolism. 90:247-255.

Basal medium (contains no growth supplement).В  Add GS before use. 315-500 6602.41 руб.

All-in-one ready-to-use 316-500 11460.8 руб.

Basal Medium and Growth Supplement sold together, bottled separately 316K-500 12457.48 руб.

Added to Basal Medium to create Growth Medium 316-GS 5855.07 руб.

HCF Total Kit: Media, Subculture Reagents & Cells, Adult 306K-05a 90691.07 руб.
HCF Total Kit: Media, Subculture Reagents & Cells, Fetal 306K-05f 90691.07 руб.

Cryopreserved HCF 306-05a 74122.54 руб.

Cryopreserved HCF 306-05f 74122.54 руб.

Human FGF9 ELISA Kit CL0348 52944.28 руб.

Total RNA prepared from Human Cardiac Fibroblasts, adult 306-R10a 47338.55 руб.
Total RNA prepared from Human Cardiac Fibroblasts, adult 306-R25a 94677.11 руб.
Total RNA prepared from Human Cardiac Fibroblasts, fetal 306-R10f 40486.8 руб.
Total RNA prepared from Human Cardiac Fibroblasts, fetal 306-R25f 80974.29 руб.

Total RNA prepared from human heart tissue 1H30-50 16039.28 руб.
Total RNA prepared from human heart tissue 1H30-250 60419.04 руб.

Proliferating HCF 307-25a 74122.54 руб.
Proliferating HCF 307-75a 97791.48 руб.
Proliferating HCF 307-6Wa 97791.48 руб.
Proliferating HCF 307-96Wa 112740.99 руб.

Proliferating HCF 307-25f 74122.54 руб.
Proliferating HCF 307-75f 97791.48 руб.
Proliferating HCF 307-6Wf 97791.48 руб.
Proliferating HCF 307-96Wf 112740.99 руб.

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K 6353.07 руб.

Extended Family Products

100 tests 028-S 4152.49 руб.
500 tests 028-01 16070.53 руб.

25 x 24-Well Rxns TF103KS 6222.62 руб.
250 x 24-Well Rxns TF103K 46715.54 руб.

Human Fibroblast Growth Factor acidic RP1029-10 9343.11 руб.
Human Fibroblast Growth Factor acidic RP1029-1000 180634.8 руб.
Human Fibroblast Growth Factor acidic RP1029-50 22423.6 руб.

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