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Anti-eEF2: Mouse eEF2 Antibody

BACKGROUND Ribosomal protein synthesis is one of the oldest and best-conserved processes taking place in a living cell. Amino acid residues are added to the growing peptide chain in the ribosome by an elongation process that involves two GTP-switched elongation factors, denominated EF1 and EF2 in eukaryotes. EF1-GTP brings the aminoacyl-tRNA (as the so-called ternary complex) to the acceptor site on the ribosome. After the nascent protein chain is transpeptidated to the newly arrived tRNA, EF2 catalyzes a conformational switch of the organelle, such that the newly generated peptidyl-tRNA is moved from the acceptor site to the peptidyl site, liberating the former for a new round of elongation. EF2 is a large (more than 800-residue), probably multifunctional, and remarkable protein that apparently binds to the same ribosomal structures as the EF1-GTP–aminoacyl-tRNA complex.

eEF2 (eukaryotic translation elongation factor 2) is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. eEF2 is phosphorylated and inhibited by a calcium/calmodulin-dependent protein kinase called eEF2 kinase, which modifies Thr56 and Thr58. Phosphorylation at Thr56 and Thr58 results in the inactivation of eEF2 by causing a structural alteration that reduces its affinity for the ribosome, thereby preventing its ability to catalyze translocation.1 eEF2 kinase is also subjected to regulation by phosphorylation, and a number of phosphorylation sites have been identified that lead to subsequent activation or inhibition of activity.2,3
1. Redpath, N.T. et al: Eur. J. Biochem. 213:689-99, 1993
2. Knebel, A. et al: EMBO J. 20:4360-9, 2001
3. Rose, A.J. et al: J. Physiol. 587:1547-63, 2009
Products are for research use only. They are not intended for human, animal, or diagnostic applications.


Raised against purified recombinant fragments of human eEF2 expressed in E. Coli.
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Applications &
Suggested starting
WB                  1:1000
IP                    n/d
IHC                  1:50 - 1:200
ICC                  1:50 - 1:200
FACS               n/d
Predicted Molecular
Weight of protein:
95 kDa
Detects eEF2 proteins without cross-reactivity with other family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.


Mouse eEF2 Antibody CP10375 34999.97 руб.

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