BACKGROUND Methionine adenosyltransferase (MAT, ATP:L-methionine Sadenosyltransferase, EC 18.104.22.168) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP and thus plays a central role in cellular metabolism. The the adenosyl moiety of ATP is transferred to methionine, forming a highly energetic compound as a result of a sulphonium bond between the 5’-carbon atom of the ribose and the sulphur atom of the amino acid. There are two isoenzymes of MAT: one is liver-specific and the other is present in all tissues. The first has a relatively high Km for methionine and the second has a low Km for methionine.1 AdoMet acts as the methyl donor for over 115 different cellular transmethylation reactions, including those of DNA, RNA, proteins, and lipids. DNA methylation is a important epigenetic feature of DNA that plays a critical role in gene expression and regulation. In addition, AdoMet participates in the transsulfuration pathway and, after decarboxylation, serves as a propylamine group donor in the biosynthesis of polyamines.2 Moreover, with the transfer of methyl group, AdoMet is converted into S-adenosylhomocysteine (AdoHcy). The majority of AdoMet-dependent methyltransferases are strongly inhibited by AdoHcy. The intracellular AdoMet/AdoHcy ratio has been used as a predictor of cellular methylation activity. AdoHcy is further converted into homocysteine and adenosine by the AdoHcy hydrolase, which is widely distributed in mammalian tissues. Hypercysteinaemia has been regarded as a new modifiable risk factor for atherosclerosis and vascular diseases.3
1. Castro, R. et al: J. Inherit. Metb. Dis. 29:3-20, 2006
2. Ubagai, T. et al: J. Clin. Invest. 96:1943-7, 1995
3. Fowler, B.: Semin. .Vascul. Med. 5:77-86, 2005
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Raised against recombinant human MAT/MATN1 fragments expressed in E. coli.
Species & predicted
reactivity ( ):
Human, Mouse, Rat
ICC 1:50 - 1:200
Weight of protein:
Detects endogenous MAT/MATN1 proteins without cross-reactivity with other family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.a