Anti-TFIIB: Monoclonal Transcription Factor II B Antibody (48C6) |
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BACKGROUND Transcription Factor II B (TF IIB) is an essential component of the RNA polymerase II (Pol II) machinery that acts as a bridge between the TATA-binding protein (TBP) and Pol II.1 The C-terminal domain of TFIIB interacts with the TBP-TATA complex, whereas the N-terminal domain is required for the assembly of TFIIF and Pol II into the preinitiation complex. It is involved in the selection of the transcription start site: mutations in TFIIB cause a shift in the transcription start site.2 In addition, TFIIB interacts with certain TBP-associated factors (TAFs) in vitro, and these interactions might contribute to recruitment or stable association of TFIIB in the context of an active transcription complex.3
REFERENCES
1. Ha, I. et al: Nature 352:689, 1991.
2. Lee, T. I. & Young, R. A. : Annu. Rev. Genet. 34:77, 2000.
3. Goodrich, J. A. et al: Cell 75:519, 1993.
2. Lee, T. I. & Young, R. A. : Annu. Rev. Genet. 34:77, 2000.
3. Goodrich, J. A. et al: Cell 75:519, 1993.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat.No.: | CB14574 |
Antigen: | E. coli-expressed recombinant human TFIIB protein fragments. |
Isotype: | Mouse monoclonal IgG2b |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 38 kDa |
Specificity/Sensitivity: | Specifically detects endogenous levels of human TFIIB proteins. Does not cross-react with other related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
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