BACKGROUND Transforming growth factor alpha (TGF alpha) is a 6 kDa polypeptide mitogen that interacts with the epidermal growth factor receptor and activates its intrinsic tyrosine kinase. The mature 50 amino acid TGF alpha is released from a 159 or 160 amino acid integral membrane glycoprotein precursor, denoted proTGF alpha, via cleavage at both termini by protease with elastase-like specificity.1 Expression of TGF alpha is most prevalent and abundant in transformed cells and tumors, but also detectable at modest levels in a limited number of normal cells and tissues. In addition, TGF-alpha is transiently expressed in some fetal and adjacent maternal tissues during development. Tthis pattern of expression suggests that the growth factor is involved in several distinct physiological functions.2 In many neoplastic cells, proteolytic processing of proTGF alpha is incomplete and/or inefficient, resulting in the preponderance of soluble and/or membrane-bound forms larger than the mature TGF alpha. Moreover, TGF-alpha and the EGF receptor are coexpressed in a number of human and rodent tumors and tumor cell lines--which suggests that TGF-alpha can function as an autocrine or paracrine growth factor.3
1. Salomon, D.S. et al: Cancer Cells. 2:389-97, 1990
2. Kudlow, J.E. & Bjorge J.D.: Semin. Cancer Biol. 1:293-302, 1990
3. Kumar, V. et al: Cell Biol. Int. 19:373-88, 1995
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Target Protein Species:
No detectable cross-reactivity
with any other cytokine.
Store at 4°C. Use within 6 months.
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.