Leptin: Human Leptin |
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BACKGROUND Leptin a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.
Recombinant human Leptin is a non-glycosylated protein containing 147 amino acids and having a molecular mass of 16 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Параметры
Cat. No.:
RP1037
Alternative Name:
Obesity protein
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human Leptin is lyophilized with no additives.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is determined by the leptin-dependent stimulation of Human OB-R transfected mouse BaF/3 proliferation and is typically 0.4-2 ng/ml.
AA Sequence:
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
Документы
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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой
