BACKGROUND Monocyte Chemoattractant Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells.
Recombinant mouse MCP-1 is a non-glycosylated protein, containing 125 amino acids and having a molecular mass of 13.8 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
CCL2, JE, MCAF
Sterile Filtered white lyophilized (freeze-dried) powder.
Recombinant mouse MCP-1 is lyophilized with no additives.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
The activity is determined by its ability to chemoattract human monocytes at 4 - 15 ng/ml.
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