BACKGROUND The enhancer of zeste homolog 2 (EZH2) is a member of the polycomb group of genes (PcG), which are important for transcriptional regulation through nucleosome modification, chromatin remodeling, and interaction with other transcription factors. EZH2 is the catalytic subunit of Polycomb repressive complex 2 (PRC2) also including EED and SUZ2, which is a highly conserved histone methyltransferase that targets lysine-27 of histone H3.1 This methylated H3-K27 chromatin mark is commonly associated with silencing of differentiation genes in organisms ranging from plants to flies to humans. Disruption of EZH2 expression may lead to dysregulation of genes critical for the G2-M transition.2 Studies on human tumors show that EZH2 is frequently over-expressed in a wide variety of cancerous tissue types, including prostate and breast.3 Although the mechanistic contributions of EZH2 to cancer progression are not yet determined, functional links between EZH2-mediated histone methylation and DNA methylation suggest partnership with the gene silencing machinery implicated in tumor suppressor loss. EZH2 expression itself is controlled by E2F transcription factors, which are downstream of the retinoblastoma protein (Rb), and is also involved in p53-regulated cell cycle control.4
1. Chen X et al.: Mol. Cell, 32:491-502, 2008.
2. Burdach S et al.: Cell Cycle 8:1991-6, 2009.
3. Backmann IM et al.: J. Clin. Onco. 24: 268-273, 2006.
4. Tang X et al.: Oncogene 23:5759-69, 2004.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Recombinant human EZH2 protein expressed in E. coli.
Mouse monoclonal IgG1
Species & predicted
reactivity ( ):
Human, Mouse, Rat
IHC (Paraffin) n/d
Weight of protein:
Detects endogenous levels of EZH2 proteins in various cell lysates.
Store at 4° C for frequent use; at -20° C for at least one year.
*Optimal working dilutions must be determined by end user.