Грам-негативные бактерии |
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Gram-negative bacteria are characterized by a lipopolysaccharide-containing outer cell membrane with 3-Deoxy-D-manno-octulosonic acid (KDO) as an essential component.
The Azide-functionalized 3-Deoxy-D-manno-octulosonic acid (KDO-Azide) provides an attractive approach for the detection of gram-negative bacteria in living cultures[1]: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart KDO.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of (Desthio)Biotin or DBCO-containing (Desthio)Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of Fluorescent Dyes or DBCO-containing Fluorescent Dyes , respectively) for subsequent microscopic imaging.
Tested bacterial strains[1]:
Legionella pneumophila, Salmonella typhimurium, Escherichia coli O86, Escherichia coli K12
[1] Dumont et al. (2012) Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core. Angew. Chem. Int. Ed. 51:3143.
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