DMEM высоко-глюкозная среда, набор
250 мл среды, базовой
12.5 ml hPL supplement (5% final), deep-frozen
DMEM (Dulbecco's modification of MEM) cell culture medium supplemented with hPL (human Platelate lysate)
DMEM contains four-fold concentrations of the amino acids and vitamins as compared to BME (Basal Medium Eagle). This culture medium, enriched with 4.5 g/L glucose, supports the proliferation of a wide variety of normal and transformed cells, such as primary cultures of mouse and chicken cells growing adherently.
The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Contrary to FBS, hPL is a bystander product. Platelet preparations must be destroyed 5 days after collection from the donors unless they are used up in other applications. Only platelets from double-negative tested donors are used to manufacture the platelet lysates and are pooled from 25 donors.
DMEM supplemented with hPL and high glucose delivers sufficient nutrients and growth factors to sustain the proliferation of cell lines such as HaCaT keratinocyte cells and U-251 MG astrocytic carcinoma cells.
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